An optimized two-step chromatin immunoprecipitation protocol to quantify the associations of two separate proteins and their common target DNA.
STAR Protoc
; 2(2): 100504, 2021 06 18.
Article
em En
| MEDLINE
| ID: mdl-33997818
ABSTRACT
Sequential chromatin immunoprecipitation (ChIP) is commonly used to investigate DNA-protein and protein-protein interactions to a specific genomic region. However, it can be tricky to achieve a robust and reproducible signal with sequential ChIP. Here, we provide an optimized two-step ChIP protocol to quantify the in vivo associates of multiple proteins with the same DNA regulatory element. For complete details on the use and execution of this protocol, please refer to He et al. (2020).
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Elementos de Resposta
/
Imunoprecipitação da Cromatina
/
Proteínas de Ligação a DNA
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China