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Impact of RNA degradation on influenza diagnosis in the surveillance system.
Bai, Hongyan; Zhao, Jiashen; Ma, Chunyan; Wei, Hejiang; Li, Xiyan; Fang, Qiongqiong; Yang, Peng; Wang, Quanyi; Wang, Dayan; Xin, Li.
Afiliação
  • Bai H; Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Centers for Disease Control and Prevention, WHO Collaborating Center for Reference and Research on Influenza, Key Laboratory for Medical Virology, National Health Commission, Beijing, People's Rep
  • Zhao J; Beijing Center for Disease Prevention and Control, Beijing, People's Republic of China.
  • Ma C; Beijing Center for Disease Prevention and Control, Beijing, People's Republic of China.
  • Wei H; Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Centers for Disease Control and Prevention, WHO Collaborating Center for Reference and Research on Influenza, Key Laboratory for Medical Virology, National Health Commission, Beijing, People's Rep
  • Li X; Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Centers for Disease Control and Prevention, WHO Collaborating Center for Reference and Research on Influenza, Key Laboratory for Medical Virology, National Health Commission, Beijing, People's Rep
  • Fang Q; Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Centers for Disease Control and Prevention, WHO Collaborating Center for Reference and Research on Influenza, Key Laboratory for Medical Virology, National Health Commission, Beijing, People's Rep
  • Yang P; Beijing Center for Disease Prevention and Control, Beijing, People's Republic of China.
  • Wang Q; Beijing Center for Disease Prevention and Control, Beijing, People's Republic of China.
  • Wang D; Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Centers for Disease Control and Prevention, WHO Collaborating Center for Reference and Research on Influenza, Key Laboratory for Medical Virology, National Health Commission, Beijing, People's Rep
  • Xin L; Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Centers for Disease Control and Prevention, WHO Collaborating Center for Reference and Research on Influenza, Key Laboratory for Medical Virology, National Health Commission, Beijing, People's Rep
Diagn Microbiol Infect Dis ; 100(4): 115388, 2021 Aug.
Article em En | MEDLINE | ID: mdl-34030102
BACKGROUND: The continuous evolution of influenza viruses is monitored by the World Health Organization Global Influenza Surveillance and Response System. Sample quality is essential for surveillance quality. METHODS: To evaluate the RNA degradation of clinical samples, influenza-like illness samples were collected from four sentinel hospitals, and seasonal influenza was tested by real-time reverse transcription polymerase chain reaction and quantified by digital reverse transcription polymerase chain reaction at different time points. RESULTS: RNA degradation was observed in the majority of samples eight days after sample collection. A significant and faster rate of RNA content reduction was observed in low viral load samples (<10 copies/µl) than in high viral load samples (>10 copies/µl), stored at 2 to 8°C for up to eight days. RNase P (RNP) RNA, which is a key indicator to evaluate sample collection quality, was detected. Sample collection quality was uneven in different hospitals. CONCLUSION: Low viral load samples increase the risk of false negatives due to RNA degradation to undetectable levels.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Manejo de Espécimes / RNA Viral / Carga Viral / Estabilidade de RNA / Influenza Humana / Vírus da Influenza A Subtipo H1N1 Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Revista: Diagn Microbiol Infect Dis Ano de publicação: 2021 Tipo de documento: Article País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Manejo de Espécimes / RNA Viral / Carga Viral / Estabilidade de RNA / Influenza Humana / Vírus da Influenza A Subtipo H1N1 Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Revista: Diagn Microbiol Infect Dis Ano de publicação: 2021 Tipo de documento: Article País de publicação: Estados Unidos