Gene Expression and Survival of Acute Lymphoblastic Leukemia Cells After Allogeneic Transplant.
Anticancer Res
; 41(6): 2781-2793, 2021 Jun.
Article
em En
| MEDLINE
| ID: mdl-34083268
ABSTRACT
BACKGROUND/AIM:
This study explored the mechanisms of the allogeneic graft versus leukemia effect in acute lymphoblastic leukemia (ALL) cells by examining whether they change gene expression in the post-transplant environment containing cytokines and the immunosuppressant cyclosporine, and if such changes affect ALL cell survival. MATERIALS ANDMETHODS:
RNASeq was used to assess leukemia global gene expression and flow cytometry to measure ALL survival in the presence of T cells, NK cells, cytokines, and cyclosporine.RESULTS:
A total of 4,805 genes were differentially expressed. Gene set enrichment analysis demonstrated up-regulation of biological processes related to cytokine responses, control of viral infection, and regulation of leukocyte function including proliferation. Down-regulated genes were related to mesenchymal tissue morphogenesis. ALL cells exposed to cytokines and cyclosporine retained susceptibility to T and NK cell killing, and also exhibited increased cell death without exposure to killer cells.CONCLUSION:
A significant portion of the graft versus leukemia effect may be mediated by cytokines and cyclosporine.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
/
Sobrevivência Celular
/
Transplante de Células-Tronco Hematopoéticas
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Leucemia-Linfoma Linfoblástico de Células Precursoras
Limite:
Adult
/
Animals
/
Child
/
Humans
Idioma:
En
Revista:
Anticancer Res
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos