Generation of 3'UTR knockout cell lines by CRISPR/Cas9-mediated genome editing.

Mitschka, Sibylle; Fansler, Mervin M; Mayr, Christine

Mitschka, Sibylle; Fansler, Mervin M; Mayr, Christine.

Afiliação

Mitschka S; Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, NY, United States.
Fansler MM; Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, NY, United States; Tri-Institutional Training Program in Computational Biology and Medicine, Weill-Cornell Graduate College, New York, NY, United States.
Mayr C; Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, NY, United States; Tri-Institutional Training Program in Computational Biology and Medicine, Weill-Cornell Graduate College, New York, NY, United States. Electronic address: mayrc@mskcc.org.

Methods Enzymol ; 655: 427-457, 2021.

Article em En | MEDLINE | ID: mdl-34183132

Assuntos

Sistemas CRISPR-Cas; Edição de Genes; Regiões 3' não Traduzidas; Linhagem Celular; Humanos; Poliadenilação

Palavras-chave

3' Untranslated region; 3'UTR knockout; APA; Alternative polyadenylation; CRISPR/Cas9-mediated deletion; Gene editing; Manipulation of non-coding elements; Post-transcriptional gene regulation

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sistemas CRISPR-Cas / Edição de Genes Limite: Humans Idioma: En Revista: Methods Enzymol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos

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