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Label-retention expansion microscopy.
Shi, Xiaoyu; Li, Qi; Dai, Zhipeng; Tran, Arthur A; Feng, Siyu; Ramirez, Alejandro D; Lin, Zixi; Wang, Xiaomeng; Chow, Tracy T; Chen, Jiapei; Kumar, Dhivya; McColloch, Andrew R; Reiter, Jeremy F; Huang, Eric J; Seiple, Ian B; Huang, Bo.
Afiliação
  • Shi X; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA.
  • Li Q; Department of Developmental and Cell Biology, University of California, Irvine, Irvine, CA.
  • Dai Z; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA.
  • Tran AA; Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA.
  • Feng S; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA.
  • Ramirez AD; Graduate Program in Chemistry and Chemical Biology, University of California, San Francisco, San Francisco, CA.
  • Lin Z; University of California, Berkeley-University of California, San Francisco Joint Graduate Program in Bioengineering, University of California, San Francisco, San Francisco, CA.
  • Wang X; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA.
  • Chow TT; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA.
  • Chen J; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA.
  • Kumar D; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA.
  • McColloch AR; Department of Pathology, University of California, San Francisco, San Francisco, CA.
  • Reiter JF; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA.
  • Huang EJ; Department of Developmental and Cell Biology, University of California, Irvine, Irvine, CA.
  • Seiple IB; Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA.
  • Huang B; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA.
J Cell Biol ; 220(9)2021 09 06.
Article em En | MEDLINE | ID: mdl-34228783
Expansion microscopy (ExM) increases the effective resolving power of any microscope by expanding the sample with swellable hydrogel. Since its invention, ExM has been successfully applied to a wide range of cell, tissue, and animal samples. Still, fluorescence signal loss during polymerization and digestion limits molecular-scale imaging using ExM. Here, we report the development of label-retention ExM (LR-ExM) with a set of trifunctional anchors that not only prevent signal loss but also enable high-efficiency labeling using SNAP and CLIP tags. We have demonstrated multicolor LR-ExM for a variety of subcellular structures. Combining LR-ExM with superresolution stochastic optical reconstruction microscopy (STORM), we have achieved molecular resolution in the visualization of polyhedral lattice of clathrin-coated pits in situ.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoblastos / Coloração e Rotulagem / Células-Tronco Embrionárias Murinas / Microscopia de Fluorescência / Microtúbulos Limite: Animals / Humans Idioma: En Revista: J Cell Biol Ano de publicação: 2021 Tipo de documento: Article País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoblastos / Coloração e Rotulagem / Células-Tronco Embrionárias Murinas / Microscopia de Fluorescência / Microtúbulos Limite: Animals / Humans Idioma: En Revista: J Cell Biol Ano de publicação: 2021 Tipo de documento: Article País de publicação: Estados Unidos