A pooled CRISPR/AsCpf1 screen using paired gRNAs to induce genomic deletions in Chinese hamster ovary cells.
Biotechnol Rep (Amst)
; 31: e00649, 2021 Sep.
Article
em En
| MEDLINE
| ID: mdl-34277363
AsCpf1, Cpf1 from Acidaminococcus sp BV3L6; CHO, Chinese hamster ovary; CPM, counts per million reads mapped; CRISPR, Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR/AsCpf1; Cas9, CRISPR-associated protein 9; Chinese hamster ovary cells; Cpf1, CRISPR-associated protein in Prevotella and Francisella; DE, differentially expressed; DOWN-TTS, downstream transcription termination site; DR, differentially represented; EV, empty vector; EpoFc, Erythropoietin Fc fusion protein; FACS, fluorescence activated cell sorting; FC, fold change; FDR, false discovery rate; GS, glutamine synthetase; Genetic screen; NGS, next generation sequencing; NTC, no template control; PAM, protospacer adjacent motif; PCA, principal component analysis; Qp, specific productivity; RMCE, recombinase-mediated cassette exchange; TMM, trimmed mean of M values; UP-TSS, upstream transcription start site; VCD, viable cell density; dCas9, deactivated Cas9; gRNA, guide RNA; genomic deletion; lncRNA, long non-coding RNA; ncGene, non-coding gene; oligo, oligonucleotide; paired gRNAs; pgRNA, paired gRNA; sgRNA, single guide RNA; µ, growth rate
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Biotechnol Rep (Amst)
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Áustria
País de publicação:
Holanda