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Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2.
Zhang, Lin; Jiang, Wenming; Zhang, Fuyou; Li, Yang; Li, Jinping; Liang, Shaobo; Yu, Xiaohui; Peng, Cheng; Liu, Shuo; Wang, Jingjing; Sun, Shuhong; Liu, Hualei.
Afiliação
  • Zhang L; Shandong Agricultural University, Tai'an, Shandong, China.
  • Jiang W; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
  • Zhang F; Shandong Agricultural University, Tai'an, Shandong, China.
  • Li Y; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
  • Li J; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
  • Liang S; Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, China.
  • Yu X; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
  • Peng C; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
  • Liu S; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
  • Wang J; China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
  • Sun S; Shandong Agricultural University, Tai'an, Shandong, China. ssh6811@163.com.
  • Liu H; China Animal Health and Epidemiology Center, Qingdao, Shandong, China. liuhualei@cahec.cn.
Virus Genes ; 57(5): 453-458, 2021 Oct.
Article em En | MEDLINE | ID: mdl-34312741
In this study, we developed a real-time quantitative polymerase chain reaction (qPCR) assay based on a dual-labeled hydrolysis probe to simultaneously detect both duck circovirus (DuCV) 1 and DuCV-2. The reproducibility, sensitivity and specificity of the primer set and probe were evaluated using other duck pathogens. The detection limit was 20 copies per µL. The intra-assay coefficients of variation (CVs) were ≤ 0.73% and the inter-assay CVs were ≤ 1.89%. No cross-reaction occurred with other duck pathogens. In addition, the qPCR assay was successfully applied to the simultaneous detection of DuCV-1 and DuCV-2 in clinical field samples. Therefore, this assay will be useful for laboratory diagnosis and epidemiological field studies of DuCV.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Aves Domésticas / Reação em Cadeia da Polimerase / Circovirus / Infecções por Circoviridae Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Virus Genes Assunto da revista: BIOLOGIA MOLECULAR / VIROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Aves Domésticas / Reação em Cadeia da Polimerase / Circovirus / Infecções por Circoviridae Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Virus Genes Assunto da revista: BIOLOGIA MOLECULAR / VIROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos