Your browser doesn't support javascript.
loading
Oligosaccharide Metabolism and Lipoteichoic Acid Production in Lactobacillus gasseri and Lactobacillus paragasseri.
Shiraishi, Tsukasa; Maeno, Shintaro; Kishi, Sayoko; Fujii, Tadashi; Tanno, Hiroki; Hirano, Katsuaki; Tochio, Takumi; Tanizawa, Yasuhiro; Arita, Masanori; Yokota, Shin-Ichi; Endo, Akihito.
Afiliação
  • Shiraishi T; Department of Microbiology, Sapporo Medical University School of Medicine, Sapporo 060-8556, Hokkaido, Japan.
  • Maeno S; Department of Food, Aroma and Cosmetic Chemistry, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri 099-2493, Hokkaido, Japan.
  • Kishi S; Department of Food, Aroma and Cosmetic Chemistry, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri 099-2493, Hokkaido, Japan.
  • Fujii T; B Food Science Co., Ltd., Chita 478-0046, Aichi, Japan.
  • Tanno H; Department of Food, Aroma and Cosmetic Chemistry, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri 099-2493, Hokkaido, Japan.
  • Hirano K; B Food Science Co., Ltd., Chita 478-0046, Aichi, Japan.
  • Tochio T; B Food Science Co., Ltd., Chita 478-0046, Aichi, Japan.
  • Tanizawa Y; Center for Information Biology, National Institute of Genetics, Mishima 411-8540, Shizuoka, Japan.
  • Arita M; Center for Information Biology, National Institute of Genetics, Mishima 411-8540, Shizuoka, Japan.
  • Yokota SI; RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Kanagawa, Japan.
  • Endo A; Department of Microbiology, Sapporo Medical University School of Medicine, Sapporo 060-8556, Hokkaido, Japan.
Microorganisms ; 9(8)2021 Jul 26.
Article em En | MEDLINE | ID: mdl-34442669
ABSTRACT
Lactobacillus gasseri and Lactobacillus paragasseri are human commensal lactobacilli that are candidates for probiotic application. Knowledge of their oligosaccharide metabolic properties is valuable for synbiotic application. The present study characterized oligosaccharide metabolic systems and their impact on lipoteichoic acid (LTA) production in the two organisms, i.e., L. gasseri JCM 1131T and L. paragasseri JCM 11657. The two strains grew well in medium with glucose but poorly in medium with raffinose, and growth rates in medium with kestose differed between the strains. Oligosaccharide metabolism markedly influenced their LTA production, and apparent molecular size of LTA in electrophoresis recovered from cells cultured with glucose and kestose differed from that from cells cultured with raffinose in the strains. On the other hand, more than 15-fold more LTA was observed in the L. gasseri cells cultured with raffinose when compared with glucose or kestose after incubation for 15 h. Transcriptome analysis identified glycoside hydrolase family 32 enzyme as a potential kestose hydrolysis enzyme in the two strains. Transcriptomic levels of multiple genes in the dlt operon, involved in D-alanine substitution of LTA, were lower in cells cultured with raffinose than in those cultured with kestose or glucose. This suggested that the different sizes of LTA observed among the carbohydrates tested were partly due to different levels of alanylation of LTA. The present study indicates that available oligosaccharide has the impact on the LTA production of the industrially important lactobacilli, which might influence their probiotic properties.
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Microorganisms Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Microorganisms Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Japão