Serumderived exosomes from house dust mitesensitized guinea pigs contribute to inflammation in BEAS2B cells via the TLR4NFκB pathway.
Mol Med Rep
; 24(5)2021 Nov.
Article
em En
| MEDLINE
| ID: mdl-34458929
ABSTRACT
Airway epithelial cells, which are the first physical defense barrier against allergens, play a pivotal role in immunity, airway inflammation and airway remodeling. The damage and dysfunction of these cells trigger the development of airway inflammatory diseases. Exosomes, which exist in various bodily fluids, mediate cellcell communication and participate in the immune response process. The present study aimed to investigate whether serum exosomes play a proinflammatory role in bronchial epithelial cells (BEAS2B cells) and, if so, explore the underlying molecular mechanisms. A guinea pig model of House dust mite (HDM)induced asthma was established by sensitizing the rodents with HDM and PBS, and serumderived exosomes were harvested. It was found that serumderived exosomes from HDMsensitized guinea pigs displayed higher levels of exosomal markers than those from controls. Additionally, western blot analysis and reverse transcriptionquantitative PCR indicated that serumderived exosomes from HDMsensitized guinea pigs carried heat shock protein 70 and triggered an inflammatory response in BEAS2B cells via the tolllike receptor 4 (TLR4)NFκB pathway. However, TAK242, an inhibitor of the expression of TLR4, blocked the activation of the TLR4NFκB pathway. These findings provided a novel mechanism for exosomemediated inflammatory responses and a new perspective for the intervention of inflammatory airway disorders.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
NF-kappa B
/
Pyroglyphidae
/
Receptor 4 Toll-Like
/
Exossomos
/
Inflamação
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2021
Tipo de documento:
Article