Implementing fluorescence enhancement, quenching, and FRET for investigating flap endonuclease 1 enzymatic reaction at the single-molecule level.

Sobhy, Mohamed A; Tehseen, Muhammad; Takahashi, Masateru; Bralic, Amer; De Biasio, Alfredo; Hamdan, Samir M

Sobhy, Mohamed A; Tehseen, Muhammad; Takahashi, Masateru; Bralic, Amer; De Biasio, Alfredo; Hamdan, Samir M.

Afiliação

Sobhy MA; Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
Tehseen M; Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
Takahashi M; Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
Bralic A; Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
De Biasio A; Leicester Institute of Structural & Chemical Biology and Department of Molecular & Cell Biology, University of Leicester, Lancaster Rd, Leicester LE1 7HB, UK.
Hamdan SM; Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Comput Struct Biotechnol J ; 19: 4456-4471, 2021.

Article em En | MEDLINE | ID: mdl-34471492

Palavras-chave

DNA Polymerase Î´; DNA Replication; FRET; Flap endonuclease; Fluorescence enhancement; Fluorescence quenching; PIFE; Single-molecule fluorescence

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Comput Struct Biotechnol J Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Arábia Saudita País de publicação: Holanda

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