A near-infrared light triggered fluormetric biosensor for sensitive detection of acetylcholinesterase activity based on NaErF4: 0.5 % Ho3+@NaYF4 upconversion nano-probe.
Talanta
; 235: 122784, 2021 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-34517642
ABSTRACT
Acetylcholinesterase (AChE), as an important neurotransmitter, is widely present in the peripheral and central nervous systems. The aberrant expression of AChE could cause diverse neurodegenerative diseases. Herein, we developed a facile and interference-free fluorimetric biosensing platform for highly sensitive AChE activity determination based on a NaErF4 0.5 % Ho3+@NaYF4 nano-probe. This nano-probe exhibits a unique property of emitting bright monochromic red (650 nm) upconversion (UC) emission under multiband (~808, ~980, and ~1530 nm) near-infrared (NIR) excitations. The principle of this detection relies on the quenching of the strong monochromic red UC emission by oxidization products of 3,3',5,5'-tetramethylbenzidine generated through AChE-modulated cascade reactions. This system shows a great sensing performance with a detection limit (LOD) of 0.0019 mU mL- 1 for AChE, as well as good specificity and stability. Furthermore, we validated the potential of the nano-probe in biological samples by determination of AChE in whole blood with a LOD of 0.0027 mU mL-1, indicating the potential application of our proposed platform for monitoring the progression of AChE-related disease.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acetilcolinesterase
/
Técnicas Biossensoriais
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Talanta
Ano de publicação:
2021
Tipo de documento:
Article