Human microglia states are conserved across experimental models and regulate neural stem cell responses in chimeric organoids.
Cell Stem Cell
; 28(12): 2153-2166.e6, 2021 12 02.
Article
em En
| MEDLINE
| ID: mdl-34536354
ABSTRACT
Microglia are resident macrophages in the brain that emerge in early development and respond to the local environment by altering their molecular and phenotypic states. Fundamental questions about microglia diversity and function during development remain unanswered because we lack experimental strategies to interrogate their interactions with other cell types and responses to perturbations ex vivo. We compared human microglia states across culture models, including cultured primary and pluripotent stem cell-derived microglia. We developed a "report card" of gene expression signatures across these distinct models to facilitate characterization of their responses across experimental models, perturbations, and disease conditions. Xenotransplantation of human microglia into cerebral organoids allowed us to characterize key transcriptional programs of developing microglia in vitro and reveal that microglia induce transcriptional changes in neural stem cells and decrease interferon signaling response genes. Microglia additionally accelerate the emergence of synchronized oscillatory network activity in brain organoids by modulating synaptic density.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Pluripotentes Induzidas
/
Células-Tronco Neurais
Limite:
Humans
Idioma:
En
Revista:
Cell Stem Cell
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Arábia Saudita