Structure features, selenylation modification, and improved anti-tumor activity of a polysaccharide from Eriobotrya japonica.
Carbohydr Polym
; 273: 118496, 2021 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-34560937
ABSTRACT
A homogeneous polysaccharide, EJP90-1, was isolated from the leaves of E. japonica by hot water extraction in this study. EJP90-1 (7702 Da) was a heteropolysaccharide mainly consisting of â5)-linked-α-L-Araf-(1â, â4)-linked-ß-D-Manp-(1â, â2,4)-linked-α-L-Rhap-(1â, â4)-linked-α-D-Xylp-(1â, â4)-linked-ß-D-Galp-(1â, â2)-linked-ß-D-Galp-(1â, â6)-linked-ß-D-Glcp-(1â, α-D-Glcp-(4â, and t-linked-α-L-Araf. EJP90-1 was found to show moderate anti-tumor activity at the cellular level. In order to improve the anti-tumor activity and the potential applications of EJP90-1, a typical sodium selenite-nitric acid (Na2SeO3-HNO3) modification on EJP90-1 was carried out. X-ray photoelectron spectroscopy (XPS) and energy dispersive spectrometer (EDS) analysis confirmed that Se was successfully introduced into the polymer chain of EJP90-1. The subsequent in vitro cytotoxicity evaluation showed the selenylation modification derivative (EJP90-1-Se) possessed significant antiproliferative activity against cancer cells (HepG2 and A549 cells) through inducing cell apoptosis. The anti-tumor activity of EJP90-1-Se was further confirmed by zebrafish models, which inhibited the proliferation and migration of HepG2 cells and the angiogenesis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Selênio
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Eriobotrya
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Neoplasias
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Antineoplásicos
Limite:
Animals
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Humans
Idioma:
En
Revista:
Carbohydr Polym
Ano de publicação:
2021
Tipo de documento:
Article