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A whole transcriptomic approach provides novel insights into the molecular basis of organophosphate and pyrethroid resistance in Anopheles arabiensis from Ethiopia.
Messenger, Louisa A; Impoinvil, Lucy Mackenzie; Derilus, Dieunel; Yewhalaw, Delenasaw; Irish, Seth; Lenhart, Audrey.
Afiliação
  • Messenger LA; Entomology Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, U.S. Centers for Disease Control and Prevention, 1600 Clifton Rd. Atlanta, GA, 30329, USA; American Society for Microbiology, 1752 N Street, NW Washington, DC, 20036, USA; Department of Disease Control, Faculty
  • Impoinvil LM; Entomology Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, U.S. Centers for Disease Control and Prevention, 1600 Clifton Rd. Atlanta, GA, 30329, USA.
  • Derilus D; Entomology Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, U.S. Centers for Disease Control and Prevention, 1600 Clifton Rd. Atlanta, GA, 30329, USA.
  • Yewhalaw D; Tropical and Infectious Diseases Research Center, Jimma University, Jimma, Ethiopia; Department of Medical Laboratory Sciences and Pathology, College of Health Sciences, Jimma University, Jimma, Ethiopia.
  • Irish S; Entomology Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, U.S. Centers for Disease Control and Prevention, 1600 Clifton Rd. Atlanta, GA, 30329, USA; President's Malaria Initiative, Entomology Branch, Division of Parasitic Diseases and Malaria, Center for Global Health,
  • Lenhart A; Entomology Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, U.S. Centers for Disease Control and Prevention, 1600 Clifton Rd. Atlanta, GA, 30329, USA. Electronic address: AJL8@cdc.gov.
Insect Biochem Mol Biol ; 139: 103655, 2021 12.
Article em En | MEDLINE | ID: mdl-34562591
The development of insecticide resistance in malaria vectors is of increasing concern in Ethiopia because of its potential implications for vector control failure. To better elucidate the specificity of resistance mechanisms and to facilitate the design of control strategies that minimize the likelihood of selecting for cross-resistance, a whole transcriptomic approach was used to explore gene expression patterns in a multi-insecticide resistant population of Anopheles arabiensis from Oromia Region, Ethiopia. This field population was resistant to the diagnostic doses of malathion (average mortality of 71.9%) and permethrin (77.4%), with pools of survivors and unexposed individuals analyzed using Illumina RNA-sequencing, alongside insecticide susceptible reference strains. This population also demonstrated deltamethrin resistance but complete susceptibility to alpha-cypermethrin, bendiocarb and propoxur, providing a phenotypic basis for detecting insecticide-specific resistance mechanisms. Transcriptomic data revealed overexpression of genes including cytochrome P450s, glutathione-s-transferases and carboxylesterases (including CYP4C36, CYP6AA1, CYP6M2, CYP6M3, CYP6P4, CYP9K1, CYP9L1, GSTD3, GSTE2, GSTE3, GSTE4, GSTE5, GSTE7 and two carboxylesterases) that were shared between malathion and permethrin survivors. We also identified nineteen highly overexpressed cuticular-associated proteins (including CYP4G16, CYP4G17 and chitinase) and eighteen salivary gland proteins (including D7r4 short form salivary protein), which may be contributing to a non-specific resistance phenotype by either enhancing the cuticular barrier or promoting binding and sequestration of insecticides, respectively. These findings provide novel insights into the molecular basis of insecticide resistance in this lesser well-characterized major malaria vector species.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Organofosfatos / Piretrinas / Transcriptoma / Mosquitos Vetores / Anopheles Tipo de estudo: Prognostic_studies Limite: Animals País/Região como assunto: Africa Idioma: En Revista: Insect Biochem Mol Biol Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA Ano de publicação: 2021 Tipo de documento: Article País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Organofosfatos / Piretrinas / Transcriptoma / Mosquitos Vetores / Anopheles Tipo de estudo: Prognostic_studies Limite: Animals País/Região como assunto: Africa Idioma: En Revista: Insect Biochem Mol Biol Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA Ano de publicação: 2021 Tipo de documento: Article País de publicação: Reino Unido