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Improved Molecular Diagnosis and Culture of the Emerging Heteropathotype Enterohemorrhagic Escherichia coli O80:H2 Using Its Non-Melibiose-Fermenting and Antibiotic-Resistance Properties.
Bizot, Etienne; Cointe, Aurélie; Smadja, Natacha; Sergentet, Delphine; Lefèvre, Sophie; Weill, François-Xavier; Levy, Corinne; Cohen, Robert; Mariani-Kurkdjian, Patricia; Bonacorsi, Stéphane.
Afiliação
  • Bizot E; Université de Paris, IAME, UMR 1137, INSERM, Paris, France.
  • Cointe A; Service de Microbiologie, Centre National de Référence Escherichia coli, AP-HP, Hôpital Robert-Debré, Paris, France.
  • Smadja N; Université de Paris, IAME, UMR 1137, INSERM, Paris, France.
  • Sergentet D; Service de Microbiologie, Centre National de Référence Escherichia coli, AP-HP, Hôpital Robert-Debré, Paris, France.
  • Lefèvre S; Service de Microbiologie, Centre National de Référence Escherichia coli, AP-HP, Hôpital Robert-Debré, Paris, France.
  • Weill FX; Laboratoire d'Étude des Microorganismes Pathogènes, French Laboratory for Shiga Toxin-Producing Escherichia coli, VetAgro Supgrid.434200.1, Campus vétérinaire, Marcy L'Etoile, France.
  • Levy C; Institut Pasteurgrid.428999.7, Unité des Bactéries Pathogènes Entériques, Centre National de Référence des Escherichia coli, Shigella et Salmonella, Paris, France.
  • Cohen R; Institut Pasteurgrid.428999.7, Unité des Bactéries Pathogènes Entériques, Centre National de Référence des Escherichia coli, Shigella et Salmonella, Paris, France.
  • Mariani-Kurkdjian P; Association Clinique Thérapeutique Infantile du Val de Marne (ACTIV), Saint Maur des Fossés, France.
  • Bonacorsi S; Association Clinique Thérapeutique Infantile du Val de Marne (ACTIV), Saint Maur des Fossés, France.
J Clin Microbiol ; 60(1): e0153021, 2022 01 19.
Article em En | MEDLINE | ID: mdl-34586892
Enterohemorrhagic Escherichia coli (EHEC) O80:H2, belonging to sequence type ST301, is among the main causes of hemolytic and uremic syndrome in Europe, a major concern in young children. Aside from the usual intimin and Shiga toxin virulence factors (VFs), this emerging serotype possesses a mosaic plasmid combining extra-intestinal VF- and antibiotic resistance-encoding genes. This hybrid pathotype can be involved in invasive infections, a rare occurrence in EHEC infections. Here, we aimed to optimize its detection, improve its clinical diagnosis, and identify its currently unknown reservoir. O80:H2 EHEC strains isolated in France between 2010 and 2018 were phenotypically and genetically analyzed and compared with non-O80 strains. The specificity and sensitivity of a PCR test and a culture medium designed, based on the molecular and phenotypic signatures of O80:H2 EHEC, were assessed on a collection of strains and stool samples. O80:H2 biotype analysis showed that none of the strains (n = 137) fermented melibiose versus 5% of non-O80 EHEC (n = 19/352). This loss of metabolic function is due to deletion of the entire melibiose operon associated with the insertion of a 70-pb sequence (70mel), a genetic scar shared by all ST301 strains. This metabolic hallmark was used to develop a real-time PCR test (100% sensitivity, 98.3% specificity) and a melibiose-based culture medium including antibiotics, characterized by 85% specificity and sensitivity for clinical specimens. These new tools may facilitate the diagnosis of this atypical clone, help the food industry to identify the reservoir and improve our epidemiological knowledge of this threatening and emerging clone.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Farmacorresistência Bacteriana / Escherichia coli Êntero-Hemorrágica / Síndrome Hemolítico-Urêmica Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Child / Child, preschool / Humans Idioma: En Revista: J Clin Microbiol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: França País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Farmacorresistência Bacteriana / Escherichia coli Êntero-Hemorrágica / Síndrome Hemolítico-Urêmica Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Child / Child, preschool / Humans Idioma: En Revista: J Clin Microbiol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: França País de publicação: Estados Unidos