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A rapid assay provides on-site quantification of tetrahydrocannabinol in oral fluid.
Yu, Hojeong; Lee, Hoyeon; Cheong, Jiyong; Woo, Sang Won; Oh, Juhyun; Oh, Hyun-Kyung; Lee, Jae-Hyun; Zheng, Hui; Castro, Cesar M; Yoo, Yeong-Eun; Kim, Min-Gon; Cheon, Jinwoo; Weissleder, Ralph; Lee, Hakho.
Afiliação
  • Yu H; Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA 02114, USA.
  • Lee H; Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
  • Cheong J; Center for Nanomedicine, Institute for Basic Science (IBS), Seoul 03722, South Korea.
  • Woo SW; Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology, Gwangju 61005, South Korea.
  • Oh J; Center for Nanomedicine, Institute for Basic Science (IBS), Seoul 03722, South Korea.
  • Oh HK; Graduate Program of Nano Biomedical Engineering (NanoBME), Advanced Science Institute, Yonsei University, Seoul 03722, South Korea.
  • Lee JH; Department of Nano Manufacturing Technology, Korea Institute of Machinery and Materials, Daejeon 34103, South Korea.
  • Zheng H; Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA 02114, USA.
  • Castro CM; Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
  • Yoo YE; Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology, Gwangju 61005, South Korea.
  • Kim MG; Center for Nanomedicine, Institute for Basic Science (IBS), Seoul 03722, South Korea.
  • Cheon J; Graduate Program of Nano Biomedical Engineering (NanoBME), Advanced Science Institute, Yonsei University, Seoul 03722, South Korea.
  • Weissleder R; Biostatistics Center, Massachusetts General Hospital, Boston, MA 02114, USA.
  • Lee H; Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA 02114, USA.
Sci Transl Med ; 13(616): eabe2352, 2021 Oct 20.
Article em En | MEDLINE | ID: mdl-34669441
ABSTRACT
Tetrahydrocannabinol (THC), the primary psychoactive ingredient of cannabis, impairs cognitive and motor function in a concentration-dependent fashion. Drug testing is commonly performed for employment and law enforcement purposes; however, available tests produce low-sensitive binary results (lateral flow assays) or have long turnaround (gas chromatography­mass spectrometry). To enable on-site THC quantification in minutes, we developed a rapid assay for oral THC analysis called EPOCH (express probe for on-site cannabis inhalation). EPOCH features distinctive sensor design such as a radial membrane and transmission optics, all contained in a compact cartridge. This integrated approach permitted assay completion within 5 min with a detection limit of 0.17 ng/ml THC, which is below the regulatory guideline (1 ng/ml). As a proof of concept for field testing, we applied EPOCH to assess oral fluid samples from cannabis users (n = 43) and controls (n = 43). EPOCH detected oral THC in all specimens from cannabis smokers (median concentration, 478 ng/ml) and THC-infused food consumers. Longitudinal monitoring showed a fast drop in THC concentrations within the first 6 hours of cannabis smoking (half-life, 1.4 hours).
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dronabinol / Detecção do Abuso de Substâncias Idioma: En Revista: Sci Transl Med Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dronabinol / Detecção do Abuso de Substâncias Idioma: En Revista: Sci Transl Med Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos