Metabolic variation in Caribbean giant barrel sponges: Influence of age and sea-depth.

ABSTRACT

The biochemical differentiation of widely distributed long-living marine organisms according to their age or the depth of waters in which they grow is an intriguing topic in marine biology. Especially sessile life forms, such as sponges, could be expected to actively regulate biological processes and interactions with their environment through chemical signals in a multidimensional manner. In recent years, the development of chemical profiling methods such as metabolomics provided an approach that has encouraged the investigation of the chemical interactions of these organisms. In this study, LC-MS based metabolomics followed by Feature-based molecular networking (FBMN) was used to explore the effects of both biotic and environmental factors on the metabolome of giant barrel sponges, chosen as model organisms as they are distributed throughout a wide range of sea-depths. This allowed the identification of differences in the metabolic composition of the sponges related to their age and depth.