Structural characterization of a water-soluble polysaccharide from Angelica dahurica and its antitumor activity in H22 tumor-bearing mice.
Int J Biol Macromol
; 193(Pt A): 219-227, 2021 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-34688677
ABSTRACT
A novel Angelica dahurica polysaccharide (ADP) with Mw of 6.09 × 103 Da was isolated. The contents of total sugar and uronic acid in ADP were 91.04% and 12.69%. The structure characteristics indicated that ADP was an acidic polysaccharide consisting of rhamnose, arabinose, galactose, glucose, mannose, glucuronic acid and galacturonic acid (0.09 0.61 1.88 1 0.14 0.63 0.03). Moreover, there were â3)-Manp-(1â, â4, 6)-Galp-(1â, â4)-Galp-(1â, â3)-Glcp-(1â, â5)-Araf-(1â, â2)-Galp-(1â in ADP with relative molar ratios of 0.320.570.290.950.710.26. In vivo experiments suggested that ADP significantly inhibited the tumor growth of mice, increased the activities of spleen lymphocytes and natural killer (NK) cells, improved the cytokine level (IL-2 and TNF-α) and the proportions of lymphocyte subsets in the peripheral blood. The tumor cell progression was arrested in the G1 phase, and the apoptosis rate of tumor cells were 7.54% and 19.32% at the dose of 100 and 200 mg/kg, which was consistent with the results of pathological observation. In summary, the study might provide a theoretical basis for the application on functional foods containing Angelica dahurica polysaccharides.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Angelica sinensis
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Neoplasias
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Antineoplásicos
Limite:
Animals
Idioma:
En
Revista:
Int J Biol Macromol
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China