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Isolation, biochemical characterization, and development of a biodegradable antimicrobial film from Cirrhinus mrigala scale collagen.
Kulkarni, Priyanka; Maniyar, Mithun; Nalawade, Megha; Bhagwat, Prashant; Pillai, Santhosh.
Afiliação
  • Kulkarni P; SVERI's College of Pharmacy, Pandharpur, Maharashtra, 413 304, India.
  • Maniyar M; SVERI's College of Pharmacy, Pandharpur, Maharashtra, 413 304, India.
  • Nalawade M; Department of Biochemistry, Shivaji University, Kolhapur, M.S., 416 004, India.
  • Bhagwat P; Department of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology, P O Box 1334, Durban, 4000, South Africa. pkbhagwat9988@gmail.com.
  • Pillai S; Department of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology, P O Box 1334, Durban, 4000, South Africa.
Environ Sci Pollut Res Int ; 29(13): 18840-18850, 2022 Mar.
Article em En | MEDLINE | ID: mdl-34704223
Collagen is a promising candidate for food and pharmaceutical applications due to its excellent biocompatibility, low antigenicity, and controlled biodegradability; however, its heavy price restricts its utilization. Fish scales generated during the processing are generally regarded as waste material and an environmental pollutant, though they are a promising source of collagen. In the present study, Cirrhinus mrigala scales were demineralized and extracted for acid-soluble collagen (ASC) using acetic acid, with a collagen yield of 2.7%. UV-Vis spectra, SDS-PAGE, FTIR analyses, and amino acid composition confirmed the type I nature of the collagen extracted. The denaturation temperature of the collagen was found to be 30.09 °C using differential scanning calorimetry (DSC). The collagen was highly soluble at acidic pH and lower NaCl concentrations while its solubility was lowered in alkaline conditions and NaCl concentrations above 0.5 M. The collagen exhibited good emulsifying potential with an emulsion activity index (EAI) and emulsion stability index (ESI) of 21.49 ± 0.22 m2 g-1 and 15.67 ± 0.13 min, respectively. Owing to the good physicochemical characteristics of the extracted collagen, collagen-chitosan-neem extract (CCN) films were prepared subsequently which showed good antimicrobial activity against Bacillus subtilis NCIM 2635, Staphylococcus aureus NCIM 2654, Escherichia coli NCIM 2832, and Pseudomonas aeruginosa NCIM 5032, suggesting the potential of collagen in the development of antimicrobial films. These results demonstrate that the collagen from fish waste could be valorized and used effectively along with chitosan and neem extract for the synthesis of novel biodegradable films with antimicrobial efficacy.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cyprinidae / Quitosana / Anti-Infecciosos Limite: Animals Idioma: En Revista: Environ Sci Pollut Res Int Assunto da revista: SAUDE AMBIENTAL / TOXICOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Índia País de publicação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cyprinidae / Quitosana / Anti-Infecciosos Limite: Animals Idioma: En Revista: Environ Sci Pollut Res Int Assunto da revista: SAUDE AMBIENTAL / TOXICOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Índia País de publicação: Alemanha