Optimisation of methodology for whole genome sequencing of Measles Virus directly from patient specimens.
J Virol Methods
; 299: 114348, 2022 01.
Article
em En
| MEDLINE
| ID: mdl-34728271
ABSTRACT
In an era of decreasing genetic diversity of Measles Virus (MeV), effective surveillance requires a higher-resolution genotyping method or whole genome sequencing (WGS) to document elimination. Through optimization of MeV WGS protocol, we developed a MeV-specific probe enrichment method that allows next generation sequencing from clinical specimens. With the probe enrichment method, 70% of specimens can be sequenced at a read depth of greater than 10 reads with minimal off-target sequences.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sequenciamento de Nucleotídeos em Larga Escala
/
Vírus do Sarampo
Tipo de estudo:
Guideline
Limite:
Humans
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Canadá
País de publicação:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS