Your browser doesn't support javascript.
loading
RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site.
George, Jasmine; Li, Yongsheng; Kadamberi, Ishaque P; Parashar, Deepak; Tsaih, Shirng-Wern; Gupta, Prachi; Geethadevi, Anjali; Chen, Changliang; Ghosh, Chandrima; Sun, Yunguang; Mittal, Sonam; Ramchandran, Ramani; Rui, Hallgeir; Lopez-Berestein, Gabriel; Rodriguez-Aguayo, Cristian; Leone, Gustavo; Rader, Janet S; Sood, Anil K; Dey, Madhusudan; Pradeep, Sunila; Chaluvally-Raghavan, Pradeep.
Afiliação
  • George J; Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Li Y; Key Laboratory of Tropical Translational Medicine of Ministry of Education, College of Biomedical Information and Engineering, Hainan Medical University, Haikou 571199, China.
  • Kadamberi IP; Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Parashar D; Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Tsaih SW; Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Gupta P; Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Geethadevi A; Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Chen C; Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Ghosh C; Department of Biological Sciences, University of Wisconsin, Milwaukee, WI 53211, USA.
  • Sun Y; Department of Pathology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Mittal S; Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Ramchandran R; Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Rui H; Department of Pathology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Lopez-Berestein G; Department of Experimental Therapeutics, University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA; Center for RNA Interference and Non-Coding RNA, University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA.
  • Rodriguez-Aguayo C; Department of Experimental Therapeutics, University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA; Center for RNA Interference and Non-Coding RNA, University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA.
  • Leone G; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Medical College of Wisconsin Cancer Center, Milwaukee, WI 53226, USA.
  • Rader JS; Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
  • Sood AK; Center for RNA Interference and Non-Coding RNA, University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA; Department of Gynecologic Oncology and Reproductive Medicine and Cancer Biology, University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX
  • Dey M; Department of Biological Sciences, University of Wisconsin, Milwaukee, WI 53211, USA.
  • Pradeep S; Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Center of Systems Molecular Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Medical College of Wisconsi
  • Chaluvally-Raghavan P; Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Center of Systems Molecular Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA; Medical College of Wisconsi
Cell Rep ; 37(5): 109934, 2021 11 02.
Article em En | MEDLINE | ID: mdl-34731628
ABSTRACT
Fragile X-related protein-1 (FXR1) gene is highly amplified in patients with ovarian cancer, and this amplification is associated with increased expression of both FXR1 mRNA and protein. FXR1 expression directly associates with the survival and proliferation of cancer cells. Surface sensing of translation (SUnSET) assay demonstrates that FXR1 enhances the overall translation in cancer cells. Reverse-phase protein array (RPPA) reveals that cMYC is the key target of FXR1. Mechanistically, FXR1 binds to the AU-rich elements (ARE) present within the 3' untranslated region (3'UTR) of cMYC and stabilizes its expression. In addition, the RGG domain in FXR1 interacts with eIF4A1 and eIF4E proteins. These two interactions of FXR1 result in the circularization of cMYC mRNA and facilitate the recruitment of eukaryotic translation initiation factors to the translation start site. In brief, we uncover a mechanism by which FXR1 promotes cMYC levels in cancer cells.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / Proteínas Proto-Oncogênicas c-myc / Proteínas de Ligação a RNA / Fator de Iniciação 4F em Eucariotos Limite: Animals / Female / Humans Idioma: En Revista: Cell Rep Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / Proteínas Proto-Oncogênicas c-myc / Proteínas de Ligação a RNA / Fator de Iniciação 4F em Eucariotos Limite: Animals / Female / Humans Idioma: En Revista: Cell Rep Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos