Coordinated Actions of Cas9 HNH and RuvC Nuclease Domains Are Regulated by the Bridge Helix and the Target DNA Sequence.

Babu, Kesavan; Kathiresan, Venkatesan; Kumari, Pratibha; Newsom, Sydney; Parameshwaran, Hari Priya; Chen, Xiongping; Liu, Jin; Qin, Peter Z; Rajan, Rakhi

Babu, Kesavan; Kathiresan, Venkatesan; Kumari, Pratibha; Newsom, Sydney; Parameshwaran, Hari Priya; Chen, Xiongping; Liu, Jin; Qin, Peter Z; Rajan, Rakhi.

Afiliação

Babu K; Department of Chemistry and Biochemistry, Price Family Foundation Institute of Structural Biology, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019, United States.
Kathiresan V; Department of Chemistry, University of Southern California, 3430 S. Vermont Ave., Los Angeles, California 90089, United States.
Kumari P; Department of Pharmaceutical Sciences, University of North Texas System College of Pharmacy, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, Texas 76107, United States.
Newsom S; Department of Chemistry and Biochemistry, Price Family Foundation Institute of Structural Biology, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019, United States.
Parameshwaran HP; Department of Chemistry and Biochemistry, Price Family Foundation Institute of Structural Biology, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019, United States.
Chen X; Department of Pharmaceutical Sciences, University of North Texas System College of Pharmacy, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, Texas 76107, United States.
Liu J; Department of Pharmaceutical Sciences, University of North Texas System College of Pharmacy, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, Texas 76107, United States.
Qin PZ; Department of Chemistry, University of Southern California, 3430 S. Vermont Ave., Los Angeles, California 90089, United States.
Rajan R; Department of Chemistry and Biochemistry, Price Family Foundation Institute of Structural Biology, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019, United States.

Biochemistry ; 60(49): 3783-3800, 2021 12 14.

Article em En | MEDLINE | ID: mdl-34757726

Assuntos

Proteína 9 Associada à CRISPR/química; Sistemas CRISPR-Cas; DNA/química; RNA Guia de Cinetoplastídeos/química; RNA/química; Streptococcus pyogenes/química; Sítios de Ligação; Proteína 9 Associada à CRISPR/genética; Proteína 9 Associada à CRISPR/metabolismo; Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas; Cristalografia por Raios X; DNA/genética; DNA/metabolismo; Clivagem do DNA; Expressão Gênica; Cinética; Simulação de Dinâmica Molecular; Mutação; Ligação Proteica; Conformação Proteica em alfa-Hélice; Conformação Proteica em Folha beta; Domínios Proteicos; Domínios e Motivos de Interação entre Proteínas; RNA/genética; RNA/metabolismo; RNA Guia de Cinetoplastídeos/genética; RNA Guia de Cinetoplastídeos/metabolismo; Streptococcus pyogenes/enzimologia; Streptococcus pyogenes/genética; Especificidade por Substrato; Termodinâmica

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Streptococcus pyogenes / DNA / RNA / RNA Guia de Cinetoplastídeos / Sistemas CRISPR-Cas / Proteína 9 Associada à CRISPR Idioma: En Revista: Biochemistry Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos

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