The protective effect of manganese superoxide dismutase from thermophilic bacterium HB27 on hydrochloric acid-induced chemical cystitis in rats.
Int Urol Nephrol
; 54(7): 1681-1691, 2022 Jul.
Article
em En
| MEDLINE
| ID: mdl-34783980
ABSTRACT
PURPOSE:
To evaluate the effects of manganese superoxide dismutase (Mn-SOD) from thermophilic bacterium HB27 (name as Tt-SOD) on chemical cystitis.METHODS:
Control and experimental rats were infused by intravesical saline or hydrochloric acid (HCl) on the first day of the experiments. Saline, sodium hyaluronate (SH) or Tt-SOD were infused intravesically once a day for three consequent days. On the fifth day, the rats were weighted and sacrificed following a pain threshold test. The bladder was harvested for histological and biochemical analyses.RESULTS:
Tt-SOD could reduce the bladder index, infiltration of inflammatory cells in tissues, serum inflammatory factors and SOD levels, mRNA expression of inflammatory factors in tissues, and increase perineal mechanical pain threshold and serum MDA and ROS levels in HCl-induced chemical cystitis. Furthermore, Tt-SOD alleviated inflammation and oxidative stress by the negative regulation of the NF-κB p65 and p38 MAPK signaling pathway.CONCLUSIONS:
Intravesical instillation of Tt-SOD provides protective effects against HCl-induced cystitis.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Superóxido Dismutase
/
Proteínas de Bactérias
/
Cistite
Limite:
Animals
Idioma:
En
Revista:
Int Urol Nephrol
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China