Detection of Viroids by RT-PCR.

Donovan, Nerida J; Chambers, Grant A; Cao, Mengji

Donovan, Nerida J; Chambers, Grant A; Cao, Mengji.

Afiliação

Donovan NJ; Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Menangle, NSW, Australia. nerida.donovan@dpi.nsw.gov.au.
Chambers GA; Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Menangle, NSW, Australia.
Cao M; National Citrus Engineering Research Centre, Citrus Research Institute, Southwest University, Chongqing, China.

Methods Mol Biol ; 2316: 143-151, 2022.

Article em En | MEDLINE | ID: mdl-34845692

ABSTRACT

ABSTRACT

Reverse transcription-polymerase chain reaction (RT-PCR) is an effective method for detecting the presence of viroids in plant tissue. Viroid RNA is converted to cDNA and amplified to detectable levels, making it a fast and useful detection tool, even when the viroid is present at low levels. Methods of viroid detection using conventional RT-PCR are described in this chapter.

Assuntos

Viroides; Primers do DNA; DNA Complementar; Doenças das Plantas; Plantas; Reação em Cadeia da Polimerase Via Transcriptase Reversa; Viroides/genética

Palavras-chave

Gel electrophoresis; Primers; Reverse transcription-polymerase chain reaction (RT-PCR); Viroid detection

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Viroides Tipo de estudo: Diagnostic_studies Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Austrália

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