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Rapid Detection of Cysticercus cellulosae by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay.
Zhang, Dejia; Qi, Yu; Cui, Yaxuan; Song, Weiyi; Wang, Xinrui; Liu, Mingyuan; Cai, Xuepeng; Luo, Xuenong; Liu, Xiaolei; Sun, Shumin.
Afiliação
  • Zhang D; College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, China.
  • Qi Y; College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, China.
  • Cui Y; College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, China.
  • Song W; College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, China.
  • Wang X; College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, China.
  • Liu M; Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun, China.
  • Cai X; Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etioloical Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricuitural Sciences, Lanzhou, China.
  • Luo X; Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etioloical Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricuitural Sciences, Lanzhou, China.
  • Liu X; Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun, China.
  • Sun S; College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, China.
Front Cell Infect Microbiol ; 11: 762472, 2021.
Article em En | MEDLINE | ID: mdl-34858877
ABSTRACT
Cysticercosis is a neglected tropical disease caused by the larvae of Taenia solium in pigs and humans. The current diagnosis of porcine cysticercosis is difficult, and traditional pathological tests cannot meet the needs of detection. This study established a UPT-LF assay for the detection of Cysticercus cellulosae. UCP particles were bound to two antigens, TSOL18 and GP50; samples were captured, and the signal from the UCP particles was converted into a detectable signal for analysis using a biosensor. Compared to ELISA, UPT-LF has higher sensitivity and specificity, with a sensitivity of 93.59% and 97.44%, respectively, in the case of TSOL18 and GP50 antigens and a specificity of 100% for both. Given its rapidness, small volume, high sensitivity and specificity, and good stability and reproducibility, this method could be used in the diagnosis of cysticercosis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Taenia solium Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Front Cell Infect Microbiol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Taenia solium Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Front Cell Infect Microbiol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China