Parity-induced changes to mammary epithelial cells control NKT cell expansion and mammary oncogenesis.
Cell Rep
; 37(10): 110099, 2021 12 07.
Article
em En
| MEDLINE
| ID: mdl-34879282
ABSTRACT
Pregnancy reprograms mammary epithelial cells (MECs) to control their responses to pregnancy hormone re-exposure and carcinoma progression. However, the influence of pregnancy on the mammary microenvironment is less clear. Here, we used single-cell RNA sequencing to profile the composition of epithelial and non-epithelial cells in mammary tissue from nulliparous and parous female mice. Our analysis indicates an expansion of γδ natural killer T-like immune cells (NKTs) following pregnancy and upregulation of immune signaling molecules in post-pregnancy MECs. We show that expansion of NKTs following pregnancy is due to elevated expression of the antigen-presenting molecule CD1d on MECs. Loss of CD1d expression on post-pregnancy MECs, or overall lack of activated NKTs, results in mammary oncogenesis. Collectively, our findings illustrate how pregnancy-induced changes modulate the communication between MECs and the immune microenvironment and establish a causal link between pregnancy, the immune microenvironment, and mammary oncogenesis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Paridade
/
Ativação Linfocitária
/
Transformação Celular Neoplásica
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Proliferação de Células
/
Células Epiteliais
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Células T Matadoras Naturais
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Glândulas Mamárias Animais
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Neoplasias Mamárias Experimentais
Limite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Cell Rep
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos