Short-Term Humoral Immune Response of the pcDNA4-G Plasmid Expressing the Bovine Ephemeral Fever Virus G Gene in BALB/c Mice.
Vet Ital
; 57(2)2021 07 27.
Article
em En
| MEDLINE
| ID: mdl-34971503
ABSTRACT
Bovine ephemeral fever (BEF) is an arthropodborne viral disease characterised by a shortterm clinical expression that can lead to significant losses in highyielding cattle and water buffaloes. In this study, we aimed to generate a recombinant plasmid expressing the glycoprotein (G) of the BEF virus (BEFV) and to stimulate a humoral immune response to this protein in BALB / c mice immunised with the recombinant plasmid. Expression of the encoded protein was demonstrated by western blotting and immunoperoxidase tests. The suitable plasmids were intramuscularly administered to BALB/c mice on days 0, 14 and 21. The antibody response in the immunised mice was measured by a plaque reduction neutralization test (PRNT) and enzymelinked immunosorbent assay (ELISA). According to BEFV ELISA, only two of the seven animals in these groups exceeded the cutoff value. A significant difference was observed in the mean OD values at 450 nm absorbance in the pcDNA4Gimmunised group when compared with those in the plasmid control group at 30 days (p < 0.05). According to PRNT50 results, a 120 (p < 0.05) antibody response was obtained at 30 days in pcDNA4G (100 µg)immunised mice, whereas this ratio was 180 (p < 0.001) in BEFVimmunised mice (1,000 PFU/0.5 ml). We conclude that the humoral immune response was stimulated in experimental mice immunised with the recombinant plasmid. However, disappointingly, the antibody response was markedly low in pcDNA4Gimmunised mice.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças dos Roedores
/
Doenças dos Bovinos
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Vírus da Febre Efêmera Bovina
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Febre Efêmera
Limite:
Animals
Idioma:
En
Revista:
Vet Ital
Ano de publicação:
2021
Tipo de documento:
Article
País de publicação:
IT
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ITALIA
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ITALY
/
ITÁLIA