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Moderate Treadmill Training Induces Limited Effects on Quadriceps Muscle Hypertrophy in Mice Exposed to Cigarette Smoke Involving Metalloproteinase 2.
Vieira Ramos, Gracielle; de Sousa Neto, Ivo Vieira; Toledo-Arruda, Alessandra Choqueta; Marqueti, Rita de Cassia; Vieira, Rodolfo P; Martins, Milton A; Salvini, Tânia F; Durigan, João Luiz Quaglioti.
Afiliação
  • Vieira Ramos G; Physical Therapy Division, University of Brasilia, Brasília, DF, Brazil.
  • de Sousa Neto IV; Department of Physical Therapy, University Paulista, Brasília, DF, Brazil.
  • Toledo-Arruda AC; Laboratory of Molecular Analysis, Graduate Program of Sciences and Technology of Health, Universidade de Brasília, Brasília, DF, Brazil.
  • Marqueti RC; Faculty of Physiotherapy, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Vieira RP; Department of Medicine Clinical (LIM 20), School of Medicine, University of São Paulo, São Paulo, Brazil.
  • Martins MA; Laboratory of Molecular Analysis, Graduate Program of Sciences and Technology of Health, Universidade de Brasília, Brasília, DF, Brazil.
  • Salvini TF; Universidade Brasil, Post-Graduation Program in Bioengineering, São Paulo, Brazil.
  • Durigan JLQ; Laboratory of Pulmonary and Exercise Immunology (LABPEI), Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE) and Nove de Julho University (UNINOVE), São Paulo, SP, Brazil.
Article em En | MEDLINE | ID: mdl-35027823
BACKGROUND: Long-term cigarette smoke (CS) induces substantive extrapulmonary effects, including musculoskeletal system disorders. Exercise training seems to protect long-term smokers against fiber atrophy in the locomotor muscles. Nevertheless, the extracellular matrix (ECM) changes in response to aerobic training remain largely unknown. Thus, we investigated the effects of moderate treadmill training on aerobic performance, cross-sectional area (CSA), fiber distribution, and metalloproteinase 2 (MMP-2) activity on quadriceps muscle in mice exposed to chronic CS. METHODS: Male mice were randomized into four groups: control or smoke (6 per group) and exercise or exercise+smoke (5 per group). Animals were exposed to 12 commercially filtered cigarettes per day (0.8 mg of nicotine, 10 mg of tar, and 10 mg of CO per cigarette). The CSA, fibers distribution, and MMP-2 activity by zymography were assessed after a period of treadmill training (50% of maximal exercise capacity for 60 min/day, 5 days/week) for 24 weeks. RESULTS: The CS exposure did not change CSA compared to the control group (p>0.05), but minor fibers in the frequency distribution (<1000 µm2) were observed. Long-term CS exposure attenuated CSA increases in exercise conditions (smoke+exercise vs exercise) while did not impair aerobic performance. Quadriceps CSA increased in mice nonsmoker submitted to aerobic training (p = 0.001). There was higher pro-MMP-2 activity in the smoke+exercise group when compared to the smoke group (p = 0.01). Regarding active MMP-2, the exercise showed higher values when compared to the control group (p = 0.001). CONCLUSION: Moderate treadmill training for 24 weeks in mice exposed to CS did not modify CSA, despite inducing higher pro-MMP-2 activity in the quadriceps muscle, suggesting limited effects on ECM remodeling. Our findings may contribute to new insights into molecular mechanisms for CS conditions.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Condicionamento Físico Animal / Doença Pulmonar Obstrutiva Crônica / Fumar Cigarros Tipo de estudo: Clinical_trials Limite: Animals / Humans / Male Idioma: En Revista: Int J Chron Obstruct Pulmon Dis Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Nova Zelândia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Condicionamento Físico Animal / Doença Pulmonar Obstrutiva Crônica / Fumar Cigarros Tipo de estudo: Clinical_trials Limite: Animals / Humans / Male Idioma: En Revista: Int J Chron Obstruct Pulmon Dis Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Nova Zelândia