Negeviruses isolated from mosquitoes in the Brazilian Amazon.
Virol J
; 19(1): 17, 2022 01 21.
Article
em En
| MEDLINE
| ID: mdl-35062977
ABSTRACT
BACKGROUND:
There are several groups of viruses including Insect Specific Viruses (ISV) such as the taxon Negevirus, a group of viruses phylogenetically related to plant viruses. Negeviruses replicate in mosquito cells, but not in vertebrate cells.METHODS:
Pools of hematophagous arthropods were inoculated in Vero and C6/36 cells. The cells were observed to detect possible cytopathic effect. Then, indirect immunofluorescence, RT-PCR, and nucleotide sequencing were performed.RESULTS:
Seven samples which presented negative results for flaviviruses, alphaviruses and bunyaviruses, but showed cytopathic effect in C6/36 cells were sequenced. We identified the occurrence of a variety of ISVs, most of them belonging to the taxon Negevirus The Brejeira, Negev, Cordoba and Wallerfield viruses, including a new virus for science, tentatively named Feitosa virus.CONCLUSIONS:
We detected negeviruses in the Amazon region, including two viruses that were isolated for the first time in Brazil Cordoba virus and the Negev virus and, a new virus for science the Feitosa virus.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus de RNA
/
Vírus de Insetos
/
Culicidae
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Virol J
Assunto da revista:
VIROLOGIA
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Brasil