A sensitive and less cytotoxic assay for identification of proliferating T cells based on bioorthogonally-functionalized uridine analogue.

Stempels, F C; de Wit, A S; Swierstra, M S; Maassen, S; Bianchi, F; van den Bogaart, G; Baranov, M V

Stempels, F C; de Wit, A S; Swierstra, M S; Maassen, S; Bianchi, F; van den Bogaart, G; Baranov, M V.

Afiliação

Stempels FC; Department of Molecular Immunology and Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, the Netherlands.
de Wit AS; Department of Molecular Immunology and Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, the Netherlands.
Swierstra MS; Department of Molecular Immunology and Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, the Netherlands.
Maassen S; Department of Molecular Immunology and Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, the Netherlands.
Bianchi F; Department of Molecular Immunology and Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, the Netherlands.
van den Bogaart G; Department of Molecular Immunology and Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, the Netherlands. Electronic address: G.van.den.Bogaart@rug.nl.
Baranov MV; Department of Molecular Immunology and Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, the Netherlands.

J Immunol Methods ; 502: 113228, 2022 03.

Article em En | MEDLINE | ID: mdl-35074315

Assuntos

Antineoplásicos; Desoxiuridina; Antineoplásicos/farmacologia; Bromodesoxiuridina/farmacologia; Proliferação de Células; Citometria de Fluxo/métodos; Coloração e Rotulagem; Linfócitos T/metabolismo; Uridina/farmacologia

Palavras-chave

Dilution-based probe; EdU; Ethynyl-uridine; T cell activation; T cell proliferation

Texto completo

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Desoxiuridina / Antineoplásicos Tipo de estudo: Diagnostic_studies Idioma: En Revista: J Immunol Methods Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Holanda País de publicação: Holanda

Texto completo

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Buscar no Google