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Potential application of simple easy-to-use insertion-deletion (InDel) markers in citrus cultivar identification.
Noda, Takahiro; Daiou, Kaoru; Mihara, Takashi; Nagano, Yukio.
Afiliação
  • Noda T; Kumamoto Prefectural Agricultural Research Center, 3801 Sakae, Koshi, Kumamoto 861-1113, Japan.
  • Daiou K; Kumamoto Prefectural Agricultural Research Center, 3801 Sakae, Koshi, Kumamoto 861-1113, Japan.
  • Mihara T; Kumamoto Prefectural Fruit Tree Research Institute, 2566 Toyofuku Matsubase-machi, Uki, Kumamoto 869-0524, Japan.
  • Nagano Y; Analytical Research Center for Experimental Sciences, Saga University, 1 Honjo-machi, Saga 840-8502, Japan.
Breed Sci ; 71(5): 601-608, 2021 Dec.
Article em En | MEDLINE | ID: mdl-35087324
We previously developed insertion-deletion (InDel) markers that distinguish three genotypes (two homozygous and one heterozygous) of diverse citrus cultivars. These InDel markers were codominant and could be clearly detected by using simple agarose gel electrophoresis. We sought to establish a method for cultivar identification using these 28 InDel markers to genotype 31 citrus cultivars. The results revealed that a minimum of 6 markers were required to identify individuals using the three-genotype classification method. Furthermore, we found that a simple method for distinguishing between two genotypes (homozygous and heterozygous) could be used to identify individuals using a minimum of 7 markers. Our findings provide a basis for the development of simple and rapid citrus cultivar identification methods.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Breed Sci Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Japão País de publicação: Japão

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Breed Sci Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Japão País de publicação: Japão