Applying selenocysteine-mediated expressed protein ligation to prepare the membrane enzyme selenoprotein S.
Methods Enzymol
; 662: 159-185, 2022.
Article
em En
| MEDLINE
| ID: mdl-35101209
ABSTRACT
The intrinsically disordered membrane-bound selenoprotein s (selenos) takes part in the protein quality control pathway, vesicle trafficking, and NF-kB signaling. The reactive selenocysteine (Sec) at the penultimate position is responsible for its enzymatic activity. We report the preparation of the soluble segment as well as the full-length selenos using expressed protein ligation. This chapter discusses the practical considerations of expressed protein ligation using selenopeptides and describes our optimized procedure for the semi-synthesis of selenos.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Selenocisteína
/
Selenoproteínas
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Estados Unidos