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Protective effects of a chemically characterized extract from solanum torvum leaves on acetaminophen-induced liver injury.
de Souza, Gabriela R; De-Oliveira, Ana Cecilia A X; Soares, Vitor; De-Souza, Thamyris Perez; Barbi, Nancy S; Paumgartten, Francisco J R; da Silva, Antonio J R.
Afiliação
  • de Souza GR; Department of Biological Sciences, National School of Public Health, Oswaldo Cruz Foundation, Rio de Janeiro.
  • De-Oliveira ACAX; Department of Biological Sciences, National School of Public Health, Oswaldo Cruz Foundation, Rio de Janeiro.
  • Soares V; Institute for Research on Natural Products, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
  • De-Souza TP; Department of Biological Sciences, National School of Public Health, Oswaldo Cruz Foundation, Rio de Janeiro.
  • Barbi NS; Department of Clinical and Toxicological Analyses, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Paumgartten FJR; Department of Biological Sciences, National School of Public Health, Oswaldo Cruz Foundation, Rio de Janeiro.
  • da Silva AJR; Institute for Research on Natural Products, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Drug Chem Toxicol ; 46(1): 122-135, 2023 Jan.
Article em En | MEDLINE | ID: mdl-35105269
ABSTRACT
Distinct parts of Solanum torvum Swartz. (Solanaceae) are popularly used for a variety of therapeutic purposes. This study determined the phytochemical composition of a phenolic fraction of S. torvum leaf aqueous extract and investigated its antioxidant and liver-protective properties. A phenolic compound-enriched fraction, or phenolic fraction (STLAE-PF) of an infusion (STLAE) of S. torvum leaves, was tested in vitro (antagonism of H2O2 in cytotoxicity and DCF assays with HepG2/C3A cells), and in vivo for antioxidant activity and protective effects against acetaminophen (APAP)-induced liver injury in mice. Thirty-eight compounds (flavonoids, esters of hydroxycinnamic acid, and chlorogenic acid isomers) were tentatively identified (high-performance liquid chromatography coupled to high-resolution electrospray mass spectrometry) in the STLAE-PF fraction. In vitro assays in HepG2/C3A cells showed that STLAE-PF and some flavonoids contained in this phenolic fraction, at noncytotoxic levels, antagonized in a concentration-dependent manner the effects of a powerful oxidant agent (H2O2). In C57BL/6 mice, oral administration of STLAE (600 and 1,200 mg/kg bw) or STLAE-PF (300 mg/kg bw) prevented the rise in serum transaminases (ALT and AST), depletion of reduced glutathione (GSH) and elevation of thiobarbituric acid reactive species (TBARs) levels in the liver caused by APAP (600 mg/kg bw, i.p.). The hepatoprotective effects of STLAE-PF (300 mg/kg bw) against APAP-caused liver injury were comparable to those of N-acetyl-cysteine (NAC 300 or 600 mg/kg bw i.p.). These findings indicate that a phenolic fraction of S. torvum leaf extract (STLAE-PF) is a new phytotherapeutic agent potentially useful for preventing/treating liver injury caused by APAP overdosing.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Solanum / Doença Hepática Induzida por Substâncias e Drogas / Doença Hepática Crônica Induzida por Substâncias e Drogas Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Drug Chem Toxicol Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Solanum / Doença Hepática Induzida por Substâncias e Drogas / Doença Hepática Crônica Induzida por Substâncias e Drogas Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Drug Chem Toxicol Ano de publicação: 2023 Tipo de documento: Article