Ibuprofen inhibits key genes involved in androgen production in theca-interstitial cells.
F S Sci
; 2(3): 230-236, 2021 Aug.
Article
em En
| MEDLINE
| ID: mdl-35199048
ABSTRACT
OBJECTIVE:
To study the effects of ibuprofen on androgen production, gene expression, and cell viability in rat theca-interstitial cells exposed to the proinflammatory stimuli interleukin-1ß (IL-1ß) and lipopolysaccharide (LPS).DESIGN:
Animal study.SETTING:
University-based research laboratory. PATIENTS/ANIMALS Theca-interstitial cells were isolated from 30 day old female Sprague Dawley rats.INTERVENTIONS:
Theca cells were cultured with pro-inflammatory media containing IL-1ß and LPS and compared with cells cultured in control media. MAIN OUTCOMEMEASURES:
Androstenedione quantification was performed on conditioned cell culture medium using liquid chromatography-mass spectrometry. Theca cell viability was assessed using PrestoBlue cell viability assay. The gene expression of Cyp17a1, Cyp11a1, and Hsd3b was analyzed using quantitative polymerase chain reaction.RESULTS:
Both proinflammatory stimuli IL-1ß and LPS increased androstenedione concentration in cell culture medium, and these effects were mitigated with ibuprofen. Both inflammatory agents in addition increased the expression of key genes involved in androgensynthesis:
Cyp17a1, Cyp11a1, and Hsd3b; the addition of ibuprofen to the culture medium inhibited these effects. Theca cell viability increased with IL-1ß and LPS. Ibuprofen inhibited the IL-1ß-mediated increase in cell viability but did not reverse the effects of LPS.CONCLUSIONS:
In conclusion, our findings support the hypothesis that many of the alterations induced by inflammatory stimuli in theca-interstitial cells are abrogated by the addition of ibuprofen.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Tecais
/
Androgênios
Limite:
Animals
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Female
/
Humans
Idioma:
En
Revista:
F S Sci
Ano de publicação:
2021
Tipo de documento:
Article