Paper based microfluidic platform for single-step detection of mesenchymal stromal cells secreted VEGF.
Anal Chim Acta
; 1199: 339588, 2022 Mar 22.
Article
em En
| MEDLINE
| ID: mdl-35227387
ABSTRACT
Low cost and user-friendly paper microfluidic devices, combined with DNA-based biosensors with binding capacities for specific molecules, have been proposed for the developing of novel platforms that ease and speed-up the process of cell secretion monitoring. In this work, we present the first cellulose microfluidic paper-based analytical device for the single-step detection of cell secreted Vascular Endothelial Growth Factor through a self-reporting Structure Switching Signaling Aptamer. A three-part Structure Switching Signaling Aptamer was designed with an aptameric sequence specific for VEGF, which provides a quantifiable fluorescent signal through the displacement of a quencher upon VEGF recognition. The VEGF biosensor was integrated in cellulose paper, enabling the homogenous distribution of the sensor in the paper substrate and the detection of as low as 0.34 ng of VEGF in 30 min through fluorescence intensity analysis. As a proof-of-concept, the biosensor was incorporated in a microfluidic paper-based analytical device format containing a VEGF detection zone and a control zone, which was applied for the detection of cell secreted VEGF in the supernatant of mesenchymal stem cells culture plates, demonstrating its potential use in cell biology research.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Técnicas Analíticas Microfluídicas
/
Células-Tronco Mesenquimais
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Anal Chim Acta
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Espanha