Interfacial behavior of Proteinase K enzyme at air-saline subphase.
J Colloid Interface Sci
; 616: 701-708, 2022 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-35247808
ABSTRACT
This study investigates the interfacial behavior of the proteinase K enzyme at air-water interface. Adsorption of enzyme on the surface was induced using saline subphase. The surface packing and stability of the enzyme was investigated using of surface pressure-area (π-A) and surface potential-area (ΔV-A) isotherms. Proteinase K enzyme forms film at air-aqueous interface and demonstrates good stability as shown through compression-decompression cycle experiments. To characterize the surface assembly morphology of the interfacial enzymes UV-vis and fluorescence spectroscopic techniques were used. The data revealed that the enzyme Langmuir monolayer has good homogeneity with no evidence of aggregates during compression. The secondary structure of the enzyme at interface was determined to be α-helix using p-polarized infrared-reflection absorption spectroscopy. This was confirmed through Circular dichroism spectra of the enzyme Langmuir-Blodgett (LB) film which showed that the major conformation present were α-helices.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Água
Idioma:
En
Revista:
J Colloid Interface Sci
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Estados Unidos