Protective and Curative Effects of Ethanol extract of Tinospora Cordifolia on Gentamicin-induced Nephrotoxicity in Rats.

Gentamicin induced acute tubular necrosis (ATN) in intrinsic variety of acute renal failure (ARF) results from oxidative stress leading to cellular lipid peroxidation. Tinospora cordifolia (Tc), locally named as 'Guluncha' is an herbal plant with medicinal value possess antioxidant property as well as significant scavenging activity in different extracts and easily available in Bangladesh. This prospective study was approved by the Institutional Review Board of Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh. This study was conducted to assess the effect of ethanol extract of Tc on gentamicin induced renal damage in rats in the department of Pharmacology, BSMMU, Dhaka, Bangladesh from January 2014 to June 2014. For this purpose, sixty rats were divided into six equal groups. Gentamicin (80mg/kg/day, 7 days) was administered and nephrotoxicity was evaluated biochemically by estimating elevated levels of serum creatinine and serum urea. Extant of lipid peroxidation was assessed by estimating renal cortical malondialdehyde (MDA) levels. The ethanol extract of Tc (200mg/kg/day) was administered with gentamicin concurrently and also consecutively to detect preventive and curative effects respectively. Statistically significant amelioration in the biochemical parameters both in serum and renal tissue suggested that active compound or compounds extracted from Tinospora cordifolia have both protective and curative effects against nephrotoxicity, though responsible active ingredient, accurate mechanism or safety profile was not confirmed by this study.