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Antimicrobial Resistance in Escherichia coli from the Broiler Farm Environment, with Detection of SHV-12-Producing Isolates.
Martínez-Álvarez, Sandra; Sanz, Susana; Olarte, Carmen; Hidalgo-Sanz, Raquel; Carvalho, Isabel; Fernández-Fernández, Rosa; Campaña-Burguet, Allelen; Latorre-Fernández, Javier; Zarazaga, Myriam; Torres, Carmen.
Afiliação
  • Martínez-Álvarez S; Department of Agriculture and Food, University of La Rioja, 26006 Logroño, Spain.
  • Sanz S; Department of Agriculture and Food, University of La Rioja, 26006 Logroño, Spain.
  • Olarte C; Department of Agriculture and Food, University of La Rioja, 26006 Logroño, Spain.
  • Hidalgo-Sanz R; Department of Agriculture and Food, University of La Rioja, 26006 Logroño, Spain.
  • Carvalho I; Department of Agriculture and Food, University of La Rioja, 26006 Logroño, Spain.
  • Fernández-Fernández R; Department of Veterinary Sciences, University of Trás-os-Montes-and Alto Douro, 5000-801 Vila Real, Portugal.
  • Campaña-Burguet A; Department of Agriculture and Food, University of La Rioja, 26006 Logroño, Spain.
  • Latorre-Fernández J; Department of Agriculture and Food, University of La Rioja, 26006 Logroño, Spain.
  • Zarazaga M; Department of Agriculture and Food, University of La Rioja, 26006 Logroño, Spain.
  • Torres C; Department of Agriculture and Food, University of La Rioja, 26006 Logroño, Spain.
Antibiotics (Basel) ; 11(4)2022 Mar 25.
Article em En | MEDLINE | ID: mdl-35453196
Antimicrobial resistance is an important One Health challenge that encompasses the human, animal, and environmental fields. A total of 111 Escherichia coli isolates previously recovered from manure (n = 57) and indoor air (n = 54) samples from a broiler farm were analyzed to determine their phenotypes and genotypes of antimicrobial resistance and integron characterization; in addition, plasmid replicon analysis and molecular typing were performed in extended-spectrum-beta-lactamase (ESBL) producer isolates. A multidrug-resistance phenotype was detected in 46.8% of the isolates, and the highest rates of resistance were found for ampicillin, trimethoprim−sulfamethoxazole, and tetracycline (>40%); moreover, 15 isolates (13.5%) showed susceptibility to all tested antibiotics. None of the isolates showed imipenem and/or cefoxitin resistance. Twenty-three of the one hundred and eleven E. coli isolates (20.7%) were ESBL producers and carried the blaSHV-12 gene; one of these isolates was recovered from the air, and the remaining 22 were from manure samples. Most of ESBL-positive isolates carried the cmlA (n = 23), tet(A) (n = 19), and aac(6')-Ib-cr (n = 11) genes. The following genetic lineages were identified among the ESBL-producing isolates (sequence type-phylogroup-clonotype): ST770-E-CH116−552 (n = 12), ST117-B2-CH45−97 (n = 4), ST68-E-CH26−382/49 (n = 3), ST68-E-CH26−49 (n = 1), and ST10992-A/B1-CH11−23/41/580 (n = 4); the latter two were detected for the first time in the poultry sector. At least two plasmid replicon types were detected in the ESBL-producing E. coli isolates, with IncF, IncF1B, IncK, and IncHI1 being the most frequently found. The following antimicrobial resistance genes were identified among the non-ESBL-producing isolates (number of isolates): blaTEM (58), aac(6')-Ib-cr (6), qnrS (2), aac(3)-II (2), cmlA (6), tet(A)/tet(B) (22), and sul1/2/3 (51). Four different gene-cassette arrays were detected in the variable region of class 1 (dfrA1-aadA1, dfrA12-aadA2, and dfrA12-orf-aadA2-cmlA) and class 2 integrons (sat2-aadA1-orfX). This work reveals the worrying presence of antimicrobial-resistant E. coli in the broiler farm environment, with ESBL-producing isolates of SHV-12 type being extensively disseminated.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Antibiotics (Basel) Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Espanha País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Antibiotics (Basel) Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Espanha País de publicação: Suíça