GC/MS-based <sup>13</sup>C metabolic flux analysis resolves the parallel and cyclic photomixotrophic metabolism of Synechocystis sp. PCC 6803 and selected deletion mutants including the Entner-Doudoroff and phosphoketolase pathways.

Schulze, Dennis; Kohlstedt, Michael; Becker, Judith; Cahoreau, Edern; Peyriga, Lindsay; Makowka, Alexander; Hildebrandt, Sarah; Gutekunst, Kirstin; Portais, Jean-Charles; Wittmann, Christoph

GC/MS-based ¹³C metabolic flux analysis resolves the parallel and cyclic photomixotrophic metabolism of Synechocystis sp. PCC 6803 and selected deletion mutants including the Entner-Doudoroff and phosphoketolase pathways.

Schulze, Dennis; Kohlstedt, Michael; Becker, Judith; Cahoreau, Edern; Peyriga, Lindsay; Makowka, Alexander; Hildebrandt, Sarah; Gutekunst, Kirstin; Portais, Jean-Charles; Wittmann, Christoph.

Afiliação

Schulze D; Institute of Systems Biotechnology, Saarland University, Saarbrücken, Germany.
Kohlstedt M; Institute of Systems Biotechnology, Saarland University, Saarbrücken, Germany.
Becker J; Institute of Systems Biotechnology, Saarland University, Saarbrücken, Germany.
Cahoreau E; Toulouse Biotechnology Institute, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
Peyriga L; MetaboHUB-MetaToul, National Infrastructure of Metabolomics & Fluxomics, Toulouse, France.
Makowka A; RESTORE, Université de Toulouse, Inserm U1031, CNRS 5070, UPS, EFS, Toulouse, France.
Hildebrandt S; Toulouse Biotechnology Institute, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.
Gutekunst K; MetaboHUB-MetaToul, National Infrastructure of Metabolomics & Fluxomics, Toulouse, France.
Portais JC; RESTORE, Université de Toulouse, Inserm U1031, CNRS 5070, UPS, EFS, Toulouse, France.
Wittmann C; Institute of Botany, Christian-Albrecht University, Kiel, Germany.

Microb Cell Fact ; 21(1): 69, 2022 Apr 22.

Article em En | MEDLINE | ID: mdl-35459213

Assuntos

Synechocystis; Aldeído Liases; Aminoácidos/metabolismo; Dióxido de Carbono/metabolismo; Cromatografia Gasosa-Espectrometria de Massas; Análise do Fluxo Metabólico; Açúcares/metabolismo; Synechocystis/metabolismo

Palavras-chave

13C metabolic flux analysis; CO2; Calvin-Benson-Bassham cycle; Cyanobacteria; Entner-Doudoroff pathway; GCMS; Glucose; Glycolytic shunt; NMR; Oxidative pentose phosphate pathway; Phosphoketolase pathway; TCA cycle, photomixotrophic growth

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Synechocystis Idioma: En Revista: Microb Cell Fact Assunto da revista: BIOTECNOLOGIA / MICROBIOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Reino Unido

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