Thermostabilizing ketoreductase ChKRED20 by consensus mutagenesis at dimeric interfaces.

Yang, Yu-Jie; Pei, Xiao-Qiong; Liu, Yan; Wu, Zhong-Liu

Yang, Yu-Jie; Pei, Xiao-Qiong; Liu, Yan; Wu, Zhong-Liu.

Afiliação

Yang YJ; CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Pei XQ; CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
Liu Y; CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
Wu ZL; CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China. Electronic address: wuzhl@cib.ac.cn.

Enzyme Microb Technol ; 158: 110052, 2022 Aug.

Article em En | MEDLINE | ID: mdl-35490547

Assuntos

Estabilidade Enzimática; Consenso; Cinética; Mutagênese; Mutagênese Sítio-Dirigida; Temperatura

Palavras-chave

Biocatalysis; Consensus; Ketoreductase; Mutagenesis; Protein engineering; Thermostability

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estabilidade Enzimática Tipo de estudo: Guideline Idioma: En Revista: Enzyme Microb Technol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos

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