A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems.

Popescu, Codruta C; Stoian, Marius C; Cucos, Lia-Maria; Coman, Anca G; Radoi, Antonio; Paun, Anca; Hadade, Niculina D; Gautier, Arnaud; Popescu, Costin-Ioan; Matache, Mihaela

Popescu, Codruta C; Stoian, Marius C; Cucos, Lia-Maria; Coman, Anca G; Radoi, Antonio; Paun, Anca; Hadade, Niculina D; Gautier, Arnaud; Popescu, Costin-Ioan; Matache, Mihaela.

Afiliação

Popescu CC; University of Bucharest, Faculty of Chemistry, Department of Organic Chemistry, Biochemistry and Catalysis, Research Centre of Applied Organic Chemistry 90-92 Panduri Street RO-050663 Bucharest Romania mihaela.matache@g.unibuc.ro.
Stoian MC; University of Bucharest, Faculty of Chemistry, Department of Organic Chemistry, Biochemistry and Catalysis, Research Centre of Applied Organic Chemistry 90-92 Panduri Street RO-050663 Bucharest Romania mihaela.matache@g.unibuc.ro.
Cucos LM; National Institute for Research and Development in Microtechnology - IMT Bucharest 126A Erou Iancu Nicolae Street 077190 Voluntari Romania.
Coman AG; Institute of Biochemistry of the Romanian Academy 296 Spl. Independentei 060031 Bucharest Romania pop@biochim.ro.
Radoi A; University of Bucharest, Faculty of Chemistry, Department of Organic Chemistry, Biochemistry and Catalysis, Research Centre of Applied Organic Chemistry 90-92 Panduri Street RO-050663 Bucharest Romania mihaela.matache@g.unibuc.ro.
Paun A; National Institute for Research and Development in Microtechnology - IMT Bucharest 126A Erou Iancu Nicolae Street 077190 Voluntari Romania.
Hadade ND; University of Bucharest, Faculty of Chemistry, Department of Organic Chemistry, Biochemistry and Catalysis, Research Centre of Applied Organic Chemistry 90-92 Panduri Street RO-050663 Bucharest Romania mihaela.matache@g.unibuc.ro.
Gautier A; Faculty of Chemistry and Chemical Engineering, Supramolecular Organic and Organometallic Chemistry Centre, ''Babes--Bolyai'' University 11 Arany Janos Str. RO-400028-Cluj-Napoca Romania.
Popescu CI; Université Clermont Auvergne, CNRS, Sigma Clermont, ICCF F-63000 Clermont-Ferrand France.
Matache M; Institute of Biochemistry of the Romanian Academy 296 Spl. Independentei 060031 Bucharest Romania pop@biochim.ro.

RSC Adv ; 10(40): 23931-23935, 2020 Jun 19.

Article em En | MEDLINE | ID: mdl-35517349

ABSTRACT

ABSTRACT

We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC). The resin was found to be highly efficient for purification of His-tagged HCV E2 glycoproteins expressed in 293T mammalian cells.

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: RSC Adv Ano de publicação: 2020 Tipo de documento: Article