Your browser doesn't support javascript.
loading
Effect of recombinant BMP-2 and erythropoietin on osteogenic properties of biomimetic PLA/PCL/HA and PHB/HA scaffolds in critical-size cranial defects model.
Senatov, Fedor; Zimina, Anna; Chubrik, Alexander; Kolesnikov, Evgeniy; Permyakova, Elizaveta; Voronin, Andrei; Poponova, Maria; Orlova, Polina; Grunina, Tatyana; Nikitin, Kirill; Krivozubov, Mikhail; Strukova, Natalia; Generalova, Maria; Ryazanova, Anna; Manskikh, Vasily; Lunin, Vladimir; Gromov, Alexander; Karyagina, Anna.
Afiliação
  • Senatov F; National University of Science and Technology "MISIS", 119049, Leninskiy pr. 4, Moscow, Russian Federation. Electronic address: Senatov@misis.ru.
  • Zimina A; National University of Science and Technology "MISIS", 119049, Leninskiy pr. 4, Moscow, Russian Federation.
  • Chubrik A; National University of Science and Technology "MISIS", 119049, Leninskiy pr. 4, Moscow, Russian Federation.
  • Kolesnikov E; National University of Science and Technology "MISIS", 119049, Leninskiy pr. 4, Moscow, Russian Federation.
  • Permyakova E; National University of Science and Technology "MISIS", 119049, Leninskiy pr. 4, Moscow, Russian Federation.
  • Voronin A; National University of Science and Technology "MISIS", 119049, Leninskiy pr. 4, Moscow, Russian Federation.
  • Poponova M; Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098, Gamaleya Str. 18, Moscow, Russia.
  • Orlova P; Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098, Gamaleya Str. 18, Moscow, Russia.
  • Grunina T; Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098, Gamaleya Str. 18, Moscow, Russia; All-Russia Research Institute of Agricultural Biotechnology, 127550, Timiryazevskaya Str. 42, Moscow, Russia.
  • Nikitin K; Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098, Gamaleya Str. 18, Moscow, Russia.
  • Krivozubov M; Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098, Gamaleya Str. 18, Moscow, Russia.
  • Strukova N; Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098, Gamaleya Str. 18, Moscow, Russia.
  • Generalova M; Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098, Gamaleya Str. 18, Moscow, Russia.
  • Ryazanova A; Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098, Gamaleya Str. 18, Moscow, Russia.
  • Manskikh V; Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098, Gamaleya Str. 18, Moscow, Russia; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia.
  • Lunin V; Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098, Gamaleya Str. 18, Moscow, Russia; All-Russia Research Institute of Agricultural Biotechnology, 127550, Timiryazevskaya Str. 42, Moscow, Russia.
  • Gromov A; Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098, Gamaleya Str. 18, Moscow, Russia.
  • Karyagina A; Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098, Gamaleya Str. 18, Moscow, Russia; All-Russia Research Institute of Agricultural Biotechnology, 127550, Timiryazevskaya Str. 42, Moscow, Russia; Belozersky Institute of Physico-C
Mater Sci Eng C Mater Biol Appl ; 135: 112680, 2022 Apr.
Article em En | MEDLINE | ID: mdl-35581092
ABSTRACT
Osteoplastic materials PLA/PCL/HA and PHB/HA and scaffolds with a highly porous structure based on them with potential applications in regenerative medicine have been obtained by solvent casting with thermopressing and salt leaching for PLA-based samples and solid-state mixing with subsequent thermopressing and salt leaching for PHB-based samples. The scaffolds were characterized by SEM-EDX, DSC, FTIR spectroscopy, mechanical tests in compression, measurement of the contact angle, in vitro studies, including loading by recombinant BMP-2 and EPO and their release kinetics, and in vivo studies on a model of regeneration of critical-sized cranial defects in mice. Biomimetic scaffolds with micropores sizes ranged from 300 to 500 µm and volume porosity of 70% imitate trabecular bone's structure and have increased hydrophilicity to achieve osteoconductive properties. Mechanical characteristics correspond to native trabecular bone. Elastic modulus - key mechanical characteristics of bone implants - showed the values of 0.15 ± 0.04 and 0.18 ± 0.08 GPa for PLA/PCL/HA and PHB/HA scaffolds, respectively. Both materials have high biocompatibility and can be used together with recombinant proteins BMP-2 and EPO. Introduction of BMP-2 leads to induction of new bone formation, introduction of EPO results in increased angiogenesis in the implantation area. The obtained scaffolds with recombinant proteins can be used as bone implants for reconstruction of defects of lightly or non-loaded bones.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteogênese / Eritropoetina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Mater Sci Eng C Mater Biol Appl Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteogênese / Eritropoetina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Mater Sci Eng C Mater Biol Appl Ano de publicação: 2022 Tipo de documento: Article