A Rapid Near-Infrared Fluorescent Probe for Cysteine Based on Isophorone and its Application in B16 Cell Imaging.
J Fluoresc
; 32(5): 1661-1667, 2022 Sep.
Article
em En
| MEDLINE
| ID: mdl-35618873
ABSTRACT
A novel near-infrared fluorescent probe SWJT-5 based on dicyanoisophorone was synthesized. It achieved the rapid (within 40 s) and discriminative detection of Cys over Hcy and GSH with a large Stokes shift (205 nm). It showed high selectivity and sensitivity for Cys, and had an obvious enhancement of fluorescence emission. The detection limit was 0.43 µM. This probe also had low background interference and little damage to biological samples. Therefore, SWJT-5 had been applied to bioimaging in living cells successfully.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cicloexanonas
/
Cisteína
/
Corantes Fluorescentes
Limite:
Animals
Idioma:
En
Revista:
J Fluoresc
Assunto da revista:
BIOFISICA
Ano de publicação:
2022
Tipo de documento:
Article