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Effect of sericin, a silk derived protein, on the amplification of Zika virus in insect and mammalian cell cultures.
Alcalá, Ana C; Contreras, Martha A; Cuevas-Juárez, Esmeralda; Ramírez, Octavio T; Palomares, Laura A.
Afiliação
  • Alcalá AC; Departamento de Medicina Molecular y Bioprocesoso, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
  • Contreras MA; Departamento de Medicina Molecular y Bioprocesoso, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
  • Cuevas-Juárez E; Departamento de Medicina Molecular y Bioprocesoso, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
  • Ramírez OT; Departamento de Medicina Molecular y Bioprocesoso, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. Electronic address: tonatiuh.ramirez@ibt.unam.mx.
  • Palomares LA; Departamento de Medicina Molecular y Bioprocesoso, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. Electronic address: laura.palomares@ibt.unam.mx.
J Biotechnol ; 353: 28-35, 2022 Jul 20.
Article em En | MEDLINE | ID: mdl-35623476
ABSTRACT
Sericin, a silk-derived non-immunogenic protein, has been used to improve cell culture performance by increasing viability, cell concentration, and promoting adherence of several cell lines. Here, we hypothesized that the properties of sericin can enhance the amplification of flaviviruses in cell cultures. The propagation of flavivirus is inefficient and limits scientific research. Zika virus (ZIKV) is an important human pathogen that has been widely studied because of its high impact on public health. There is a need to amplify Zika virus both for research and vaccine development. In this work, we show that sericin improves ZIKV amplification in insect (C6/36) and mammalian (Vero) cell cultures, and that it has a cryoprotectant capacity. Supplementation of cell culture media with sericin at 80 µg/mL resulted in a significant increase of 1 log in the concentration of ZIKV infectious particles produced from both cell lines. Furthermore, final virus yields increased between 5 and 10-fold in Vero cells and between 7 and 23-fold in C6/36 cells when sericin was supplemented, compared to control conditions. These results show that sericin is an effective supplement to increase ZIKV production by Vero and C6/36 cells. Additionally, sericin was a suitable cryoprotective agent, and hence an alternative to FBS and DMSO, for the cryopreservation of C6/36 cells but not for Vero cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sericinas / Zika virus / Infecção por Zika virus Limite: Animals / Humans Idioma: En Revista: J Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sericinas / Zika virus / Infecção por Zika virus Limite: Animals / Humans Idioma: En Revista: J Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2022 Tipo de documento: Article