Impact of optogenetic pulse design on CA3 learning and replay: A neural model.
Cell Rep Methods
; 2(5): 100208, 2022 05 23.
Article
em En
| MEDLINE
| ID: mdl-35637904
ABSTRACT
Optogenetic manipulation of hippocampal circuitry is an important tool for investigating learning in vivo. Numerous approaches to pulse design have been employed to elicit desirable circuit and behavioral outcomes. Here, we systematically test the outcome of different single-pulse waveforms in a rate-based model of hippocampal memory function at the level of mnemonic replay extension and de novo synaptic weight formation in CA3 and CA1. Lower-power waveforms with long forward or forward and backward ramps yield more natural sequence replay dynamics and induce synaptic plasticity that allows for more natural memory replay timing, in contrast to square or backward ramps. These differences between waveform shape and amplitude are preserved with the addition of noise in membrane potential, light scattering, and protein expression, improving the potential validity of predictions for in vivo work. These results inform future optogenetic experimental design choices in the field of learning and memory.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Optogenética
/
Aprendizagem
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Cell Rep Methods
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Estados Unidos