Your browser doesn't support javascript.
loading
Vitrification and Rewarming of Magnetic Nanoparticle-Loaded Rat Hearts.
Gao, Zhe; Namsrai, Baterdene; Han, Zonghu; Joshi, Purva; Rao, Joseph Sushil; Ravikumar, Vasanth; Sharma, Anirudh; Ring, Hattie L; Idiyatullin, Djaudat; Magnuson, Elliott C; Iaizzo, Paul A; Tolkacheva, Elena G; Garwood, Michael; Rabin, Yoed; Etheridge, Michael; Finger, Erik B; Bischof, John C.
Afiliação
  • Gao Z; Department of Mechanical Engineering, University of Minnesota, 111 Church St. SE., Minneapolis, MN 55455, USA.
  • Namsrai B; Department of Surgery, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN 55455, USA.
  • Han Z; Department of Mechanical Engineering, University of Minnesota, 111 Church St. SE., Minneapolis, MN 55455, USA.
  • Joshi P; Department of Mechanical Engineering, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, PA 15213, USA.
  • Rao JS; Department of Surgery, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN 55455, USA.
  • Ravikumar V; Department of Biomedical Engineering, University of Minnesota, 312 Church St. SE, Minneapolis, MN 55455, USA.
  • Sharma A; Department of Mechanical Engineering, University of Minnesota, 111 Church St. SE., Minneapolis, MN 55455, USA.
  • Ring HL; Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, 2021 6th Street S.E. Minneapolis, Minneapolis, MN 55455, USA.
  • Idiyatullin D; Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, 2021 6th Street S.E. Minneapolis, Minneapolis, MN 55455, USA.
  • Magnuson EC; Department of Mechanical Engineering, University of Minnesota, 111 Church St. SE., Minneapolis, MN 55455, USA.
  • Iaizzo PA; Department of Surgery, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN 55455, USA.
  • Tolkacheva EG; Department of Biomedical Engineering, University of Minnesota, 312 Church St. SE, Minneapolis, MN 55455, USA.
  • Garwood M; Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, 2021 6th Street S.E. Minneapolis, Minneapolis, MN 55455, USA.
  • Rabin Y; Department of Mechanical Engineering, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, PA 15213, USA.
  • Etheridge M; Department of Mechanical Engineering, University of Minnesota, 111 Church St. SE., Minneapolis, MN 55455, USA.
  • Finger EB; Department of Surgery, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN 55455, USA.
  • Bischof JC; Department of Mechanical Engineering, University of Minnesota, 111 Church St. SE., Minneapolis, MN 55455, USA.
Adv Mater Technol ; 7(3)2022 Mar.
Article em En | MEDLINE | ID: mdl-35668819
ABSTRACT
To extend the preservation of donor hearts beyond the current 4-6 h, this paper explores heart cryopreservation by vitrification-cryogenic storage in a glass-like state. While organ vitrification is made possible by using cryoprotective agents (CPA) that inhibit ice during cooling, failure occurs during convective rewarming due to slow and non-uniform rewarming which causes ice crystallization and/or cracking. Here an alternative, "nanowarming", which uses silica-coated iron oxide nanoparticles (sIONPs) perfusion loaded through the vasculature is explored, that allows a radiofrequency coil to rewarm the organ quickly and uniformly to avoid convective failures. Nanowarming has been applied to cells and tissues, and a proof of principle study suggests it is possible in the heart, but proper physical and biological characterization especially in organs is still lacking. Here, using a rat heart model, controlled machine perfusion loading and unloading of CPA and sIONPs, cooling to a vitrified state, and fast and uniform nanowarming without crystallization or cracking is demonstrated. Further, nanowarmed hearts maintain histologic appearance and endothelial integrity superior to convective rewarming and indistinguishable from CPA load/unload control hearts while showing some promising organ-level (electrical) functional activity. This work demonstrates physically successful heart vitrification and nanowarming and that biological outcomes can be expected to improve by reducing or eliminating CPA toxicity during loading and unloading.
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Adv Mater Technol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Adv Mater Technol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos