Compartmentalization of RNA Degradosomes in Bacteria Controls Accessibility to Substrates and Ensures Concerted Degradation of mRNA to Nucleotides.
Annu Rev Microbiol
; 76: 533-552, 2022 09 08.
Article
em En
| MEDLINE
| ID: mdl-35671533
ABSTRACT
RNA degradosomes are multienzyme complexes composed of ribonucleases, RNA helicases, and metabolic enzymes. RNase E-based degradosomes are widespread in Proteobacteria. The Escherichia coli RNA degradosome is sequestered from transcription in the nucleoid and translation in the cytoplasm by localization to the inner cytoplasmic membrane, where it forms short-lived clusters that are proposed to be sites of mRNA degradation. In Caulobacter crescentus, RNA degradosomes localize to ribonucleoprotein condensates in the interior of the cell [bacterial ribonucleoprotein-bodies (BR-bodies)], which have been proposed to drive the concerted degradation of mRNA to nucleotides. The turnover of mRNA in growing cells is important for maintaining pools of nucleotides for transcription and DNA replication.Membrane attachment of the E. coli RNA degradosome is necessary to avoid wasteful degradation of intermediates in ribosome assembly. Sequestering RNA degradosomes to C. crescentus BR-bodies, which exclude structured RNA, could have a similar role in protecting intermediates in ribosome assembly from degradation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polirribonucleotídeo Nucleotidiltransferase
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RNA Mensageiro
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Caulobacter crescentus
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RNA Helicases
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Estabilidade de RNA
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Endorribonucleases
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Escherichia coli
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Complexos Multienzimáticos
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Nucleotídeos
Idioma:
En
Revista:
Annu Rev Microbiol
Ano de publicação:
2022
Tipo de documento:
Article