Cell surface characteristics and DNA content of macrophages in murine bone marrow cultures. A study using simultaneous scanning electron microscopy and fluorescence microscopy.
Histochemistry
; 86(4): 433-6, 1987.
Article
em En
| MEDLINE
| ID: mdl-3570882
ABSTRACT
An instrument combining scanning electron microscopy (SEM) and light microscopy (LM) was used to study the cell surface characteristics and DNA content of macrophages in murine bone marrow cultures. After a quantitative Feulgen DNA staining, the DNA content of the individual macrophages was measured and their cell surface morphology was studied immediately thereafter with the SEM part of the instrument. The cells were divided into six groups according to the number of microvilli and/or microridges present on their surface. A proportion of macrophages showed a DNA content more than occurs in diploid cells, which could indicate a future division. No special surface morphology could be detected in this cell type.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Células da Medula Óssea
/
Macrófagos
Limite:
Animals
Idioma:
En
Revista:
Histochemistry
Ano de publicação:
1987
Tipo de documento:
Article