Bovine genital leptospirosis: Evidence of ovarian infection by Leptospira interrogans.
Vet Microbiol
; 271: 109489, 2022 Aug.
Article
em En
| MEDLINE
| ID: mdl-35738096
ABSTRACT
Leptospirosis in ruminants causes reproductive failures leading to important economic losses. This study assessed the occurrence and genetically identified Leptospira spp. in the follicular fluid (FF) of naturally infected live cows. A total of 251 asymptomatic cows from different commercial dairy herds were subjected to ovum-pick up technique for follicular fluid sampling. PCR was performed for Leptospira spp. detection and phylogenetic analysis was later implemented for sequencing. From 251 samples analyzed, 67 (26.7 %) were lipL32-PCR positive, confirming the presence of leptospiral DNA on FF. Furthermore, it was possible to amplify and sequence nine strains after secY nested-PCR. All of them were identified as L. interrogans, with 100 % of identity with strains belonging to Sejroe serogroup. Our findings reveal a high occurrence of infection of Leptospira in the ovarium of asymptomatic cows, highlighting the importance of considering the silent leptospirosis syndrome when screening animals for assisted reproductive biotechniques.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leptospira
/
Leptospira interrogans
/
Leptospirose
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Vet Microbiol
Ano de publicação:
2022
Tipo de documento:
Article