A design of red emission CDs-based aptasensor for sensitive detection of insulin via fluorescence resonance energy transfer.

ABSTRACT

We successfully designed an aptasensor based on the red emission carbon dots (R-CDs) and effectively detected insulin (INS) via fluorescence resonance energy transfer (FRET). In the process, the aptamer (apt) labeled with R-CDs (R-CDs@apt) was used as fluorescence donor and graphene oxide (GO) was used as fluorescence receptor. The successful detection due to the aptamer sequence has a certain affinity for Go and INS, while the affinity for INS is stronger than that of GO. When INS is not added to the detection system, the aptamer is adsorbed onto the surface of GO, shortening the distance between R-CDs@apt and GO, resulting in FRET and the quenching of fluorescence of R-CDs@apt. When INS was added to the detection system, the aptamer selectively bound INS and separated from the adsorption of GO, FRET gradually disappeared and the fluorescence of R-CDs@apt/GO/INS system was restored. By comparing the changes of fluorescence intensity before and after adding INS, the detection of INS was implemented. The aptasensor has a good linear curve with the detection limit of as low as 1.1 nM when the concentration of INS reached 1.3-150 nM. This method has excellent selectivity and anti-interference. Therefore, it is a potential method for detecting substances in biological fluids.